BRENDA - Enzyme Database

Alcohol dehydrogenase in Acinobacter sp. strain HO1-N: role in hexadecane and hexadecanol metabolism

Singer, M.E.; Finnerty, W.R.; J. Bacteriol. 164, 1017-1024 (1985)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.164
0.0016
-
hexadecanol
in crude extract of hexadecane-induced cells
Acinetobacter sp.
1.1.1.164
0.0028
-
hexadecanol
in crude extract of hexadecanol-induced cells
Acinetobacter sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.164
hexadecanal + NADH
Acinetobacter sp.
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
Acinetobacter sp.
?
1.1.1.164
hexadecanal + NADH
Acinetobacter sp. HO1-N
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
Acinetobacter sp. HO1-N
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.164
Acinetobacter sp.
-
-
-
1.1.1.164
Acinetobacter sp. HO1-N
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.164
0.0008
-
growth substrate of cells ethanol, assay substrate n-hexadecanol
Acinetobacter sp.
1.1.1.164
0.0011
-
growth substrate of cells palmitic acid, assay substrate n-hexadecanol
Acinetobacter sp.
1.1.1.164
0.0046
-
growth substrate of cells hexadecanol, assay substrate n-hexadecanol
Acinetobacter sp.
1.1.1.164
0.0059
-
growth substrate of cells hexadecane, assay substrate n-hexadecanol
Acinetobacter sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.164
hexadecanal + NADH
-
286098
Acinetobacter sp.
hexadecanol + NAD+
-
286098
Acinetobacter sp.
?
1.1.1.164
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286098
Acinetobacter sp.
hexadecanol + NAD+
-
286098
Acinetobacter sp.
?
1.1.1.164
hexadecanal + NADH
-
286098
Acinetobacter sp. HO1-N
hexadecanol + NAD+
-
286098
Acinetobacter sp. HO1-N
?
1.1.1.164
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286098
Acinetobacter sp. HO1-N
hexadecanol + NAD+
-
286098
Acinetobacter sp. HO1-N
?
1.1.1.164
hexadecanol + NAD+
enzyme is important for alkane dissimilation
286098
Acinetobacter sp.
hexadecanal + NADH
-
286098
Acinetobacter sp.
?
1.1.1.164
hexadecanol + NAD+
enzyme is important for alkane dissimilation
286098
Acinetobacter sp. HO1-N
hexadecanal + NADH
-
286098
Acinetobacter sp. HO1-N
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.164
NAD+
preference for
Acinetobacter sp.
1.1.1.164
NADP+
low activity
Acinetobacter sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.164
NAD+
preference for
Acinetobacter sp.
1.1.1.164
NADP+
low activity
Acinetobacter sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.164
0.0016
-
hexadecanol
in crude extract of hexadecane-induced cells
Acinetobacter sp.
1.1.1.164
0.0028
-
hexadecanol
in crude extract of hexadecanol-induced cells
Acinetobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.164
hexadecanal + NADH
Acinetobacter sp.
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
Acinetobacter sp.
?
1.1.1.164
hexadecanal + NADH
Acinetobacter sp. HO1-N
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
Acinetobacter sp. HO1-N
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.164
0.0008
-
growth substrate of cells ethanol, assay substrate n-hexadecanol
Acinetobacter sp.
1.1.1.164
0.0011
-
growth substrate of cells palmitic acid, assay substrate n-hexadecanol
Acinetobacter sp.
1.1.1.164
0.0046
-
growth substrate of cells hexadecanol, assay substrate n-hexadecanol
Acinetobacter sp.
1.1.1.164
0.0059
-
growth substrate of cells hexadecane, assay substrate n-hexadecanol
Acinetobacter sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.164
hexadecanal + NADH
-
286098
Acinetobacter sp.
hexadecanol + NAD+
-
286098
Acinetobacter sp.
?
1.1.1.164
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286098
Acinetobacter sp.
hexadecanol + NAD+
-
286098
Acinetobacter sp.
?
1.1.1.164
hexadecanal + NADH
-
286098
Acinetobacter sp. HO1-N
hexadecanol + NAD+
-
286098
Acinetobacter sp. HO1-N
?
1.1.1.164
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286098
Acinetobacter sp. HO1-N
hexadecanol + NAD+
-
286098
Acinetobacter sp. HO1-N
?
1.1.1.164
hexadecanol + NAD+
enzyme is important for alkane dissimilation
286098
Acinetobacter sp.
hexadecanal + NADH
-
286098
Acinetobacter sp.
?
1.1.1.164
hexadecanol + NAD+
enzyme is important for alkane dissimilation
286098
Acinetobacter sp. HO1-N
hexadecanal + NADH
-
286098
Acinetobacter sp. HO1-N
?