BRENDA - Enzyme Database

New routes for lignin biosynthesis defined by biochemical characterization of recombinant ferulate 5-hydroxylase, a multifunctional cytochrome P450-dependent monooxygenase

Humphreys, J.M.; Hemm, M.R.; Chapple, C.; Proc. Natl. Acad. Sci. USA 96, 10045-10050 (1999)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.B13
0.001
-
coniferaldehyde
pH 7.8, 30°C
Arabidopsis thaliana
1.14.14.B13
0.003
-
coniferyl alcohol
pH 7.8, 30°C
Arabidopsis thaliana
1.14.14.B13
1
-
ferulate
pH 7.8, 30°C
Arabidopsis thaliana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.B13
58000
-
SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.B13
coniferaldehyde + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
5-hydroxyconiferaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.14.B13
Arabidopsis thaliana
Q42600
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.B13
stem
flowering plant stem
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.14.B13
coniferaldehyde + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyconiferaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.B13
coniferyl alcohol + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyconiferyl alcohol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.B13
ferulate + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyferulate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.14.B13
CYP84A1
-
Arabidopsis thaliana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.B13
cytochrome P450
heme-thiolate (P-450) monooxygenase
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.B13
cytochrome P450
heme-thiolate (P-450) monooxygenase
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.B13
0.001
-
coniferaldehyde
pH 7.8, 30°C
Arabidopsis thaliana
1.14.14.B13
0.003
-
coniferyl alcohol
pH 7.8, 30°C
Arabidopsis thaliana
1.14.14.B13
1
-
ferulate
pH 7.8, 30°C
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.B13
58000
-
SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.B13
coniferaldehyde + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
5-hydroxyconiferaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.B13
stem
flowering plant stem
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.14.B13
coniferaldehyde + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyconiferaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.B13
coniferyl alcohol + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyconiferyl alcohol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.B13
ferulate + [reduced NADPH-hemoprotein reductase] + O2
-
743699
Arabidopsis thaliana
5-hydroxyferulate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?