BRENDA - Enzyme Database
show all sequences of 7.6.2.9

On the osmotic signal and osmosensing mechanism of an ABC transport system for glycine betaine

van der Heide, T.; Stuart, M.C.A.; Poolman, B.; EMBO J. 20, 7022-7032 (2001)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
anionic lipids
absence of phosphatidylglycerol and/or phosphatidylserine results in trapping of the protein in an inactive state
Lactococcus lactis
glycerol
at 270 mM, the amount of glycine betaine taken up after 30 seconds is 30% higher than that of the iso-osmotic control sample
Lactococcus lactis
additional information
sucrose and D-fructose have no effects on OpuA activity
Lactococcus lactis
phosphatidylethanolamine
non-bilayer lipid essential for high activity of OpuA
Lactococcus lactis
Inhibitors
Inhibitors
Commentary
Organism
Structure
K3PO4
above 200 mM, inhibits ATPase activity
Lactococcus lactis
Tetracaine
low concentrations of the cationic amphipath decrease the activation of OpuA
Lactococcus lactis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Lactococcus lactis
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K2SO4
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
K3PO4
100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity. 200 mM, stimulates ATPase activity
Lactococcus lactis
KCl
100 mM, activates OpuA instantaneous and reversible
Lactococcus lactis
Na2SO4
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
NaCl
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
Sodium phosphate
100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity
Lactococcus lactis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactococcus lactis
-
-
-
Purification (Commentary)
Commentary
Organism
affinity chromatography of solubilized membranes
Lactococcus lactis
Reaction
Reaction
Commentary
Organism
ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] = ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1]
the osmotic activation of the transporter is set by the bulk charge in the lipid headgroup region of the membrane, indicating that electrostatic interactions between lipids and the transporter are intrinsic to the osmosensing mechanism
Lactococcus lactis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.005
-
ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg
Lactococcus lactis
0.009
-
ATPase activity, pH 7.0, 50 mM K3PO4, 290 mM sucrose, 450 mosmol/kg
Lactococcus lactis
0.018
-
ATPase activity, pH 7.0, 50 mM K3PO4, 90 mosmol/kg
Lactococcus lactis
0.023
-
ATPase activity, pH 7.0, 100 mM K3PO4, 180 mosmol/kg
Lactococcus lactis
0.095
-
ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM NaCl, 450 mosmol/kg
Lactococcus lactis
0.099
-
ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM KCl, 450 mosmol/kg
Lactococcus lactis
0.117
-
ATPase activity, pH 7.0, 150 mM K3PO4, 270 mosmol/kg
Lactococcus lactis
0.124
-
ATPase activity, pH 7.0, 350 mM K3PO4, 630 mosmol/kg
Lactococcus lactis
0.146
-
ATPase activity, pH 7.0, 250 mM K3PO4, 450 mosmol/kg
Lactococcus lactis
0.148
-
ATPase activity, pH 7.0, 50 mM K3PO4, 160 mM K2SO4, 450 mosmol/kg
Lactococcus lactis
0.18
-
ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg
Lactococcus lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + glycine betaine/out
-
655328
Lactococcus lactis
ADP + phosphate + glycine betaine/in
-
-
-
-
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
anionic lipids
absence of phosphatidylglycerol and/or phosphatidylserine results in trapping of the protein in an inactive state
Lactococcus lactis
glycerol
at 270 mM, the amount of glycine betaine taken up after 30 seconds is 30% higher than that of the iso-osmotic control sample
Lactococcus lactis
additional information
sucrose and D-fructose have no effects on OpuA activity
Lactococcus lactis
phosphatidylethanolamine
non-bilayer lipid essential for high activity of OpuA
Lactococcus lactis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
K3PO4
above 200 mM, inhibits ATPase activity
Lactococcus lactis
Tetracaine
low concentrations of the cationic amphipath decrease the activation of OpuA
Lactococcus lactis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Lactococcus lactis
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K2SO4
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
K3PO4
100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity. 200 mM, stimulates ATPase activity
Lactococcus lactis
KCl
100 mM, activates OpuA instantaneous and reversible
Lactococcus lactis
Na2SO4
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
NaCl
100 mosmol/kg osmolality, stimulates OpuA activity
Lactococcus lactis
Sodium phosphate
100 mosmol/kg osmolality, pH 7.0, stimulates OpuA activity
Lactococcus lactis
Purification (Commentary) (protein specific)
Commentary
Organism
affinity chromatography of solubilized membranes
Lactococcus lactis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.005
-
ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg
Lactococcus lactis
0.009
-
ATPase activity, pH 7.0, 50 mM K3PO4, 290 mM sucrose, 450 mosmol/kg
Lactococcus lactis
0.018
-
ATPase activity, pH 7.0, 50 mM K3PO4, 90 mosmol/kg
Lactococcus lactis
0.023
-
ATPase activity, pH 7.0, 100 mM K3PO4, 180 mosmol/kg
Lactococcus lactis
0.095
-
ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM NaCl, 450 mosmol/kg
Lactococcus lactis
0.099
-
ATPase activity, pH 7.0, 50 mM K3PO4, 200 mM KCl, 450 mosmol/kg
Lactococcus lactis
0.117
-
ATPase activity, pH 7.0, 150 mM K3PO4, 270 mosmol/kg
Lactococcus lactis
0.124
-
ATPase activity, pH 7.0, 350 mM K3PO4, 630 mosmol/kg
Lactococcus lactis
0.146
-
ATPase activity, pH 7.0, 250 mM K3PO4, 450 mosmol/kg
Lactococcus lactis
0.148
-
ATPase activity, pH 7.0, 50 mM K3PO4, 160 mM K2SO4, 450 mosmol/kg
Lactococcus lactis
0.18
-
ATPase activity, pH 7.0, 200 mM K3PO4, 360 mosmol/kg
Lactococcus lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + glycine betaine/out
-
655328
Lactococcus lactis
ADP + phosphate + glycine betaine/in
-
-
-
-
Other publictions for EC 7.6.2.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749673
Teichmann
OpuF a new Bacillus compatibl ...
Bacillus infantis, Bacillus panaciterrae, no activity in Bacillus subtilis
Appl. Environ. Microbiol.
84
e01728-18
2018
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2
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2
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6
6
-
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-
750650
Ronzheimer
The GbsR family of transcript ...
Bacillus infantis, Bacillus infantis NRRL B-14911, Bacillus subtilis 168, Bacillus subtilis
Front. Microbiol.
9
2536
2018
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2
2
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1
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2
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6
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8
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1
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6
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2
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3
3
2
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1
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3
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6
-
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1
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6
-
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2
4
6
2
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751296
Ma
Biosynthesis and uptake of gl ...
Vibrio anguillarum serotype O1, Vibrio anguillarum serotype O1 MVM425
J. Microbiol.
55
44-55
2017
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1
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1
1
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4
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4
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4
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4
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2
4
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751583
Teichmann
From substrate specificity to ...
Bacillus subtilis 168, Bacillus subtilis, Bacillus subtilis JH642
Mol. Microbiol.
104
761-780
2017
-
-
-
-
1
-
-
1
1
1
-
6
-
9
-
-
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6
1
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1
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2
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2
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1
2
2
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6
-
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6
2
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1
3
6
1
-
-
734645
Bashir
Plant-derived compatible solut ...
Bacillus subtilis
Microbiology
160
2283-2294
2014
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1
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734090
Hoffmann
Osmotic control of opuA expres ...
Bacillus subtilis, Bacillus subtilis JH642
J. Bacteriol.
195
510-522
2013
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6
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4
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1
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1
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734234
Karasawa
Physicochemical factors contro ...
Lactococcus lactis, Lactococcus lactis Opu401
J. Biol. Chem.
288
29862-29871
2013
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1
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2
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734703
Gul
Functional reconstitution and ...
Escherichia coli
Mol. Membr. Biol.
30
138-148
2013
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1
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1
1
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718937
Gul
Functional characterization of ...
Lactococcus lactis
Biochemistry
51
5142-5152
2012
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1
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4
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1
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1
1
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720502
Pilonieta
A glycine betaine importer lim ...
Salmonella enterica
Mol. Microbiol.
84
296-309
2012
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1
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1
1
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718785
Du
Structures of the substrate-bi ...
Bacillus subtilis
Biochem. J.
436
283-289
2011
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1
1
4
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720258
Pittelkow
The crystal structure of the s ...
Bacillus subtilis, Bacillus subtilis JH642
J. Mol. Biol.
411
53-67
2011
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1
8
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720807
Wolters
Ligand binding and crystal str ...
Lactococcus lactis
PLoS ONE
5
e10361
2010
1
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685001
Horn
Monitoring conformational chan ...
Bacillus subtilis
Biochem. J.
412
233-244
2008
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687343
Dreux
Glycine betaine improves Liste ...
Listeria monocytogenes, Listeria monocytogenes LO28
J. Appl. Microbiol.
104
1221-1227
2008
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698590
Smits
The compatible-solute-binding ...
Bacillus subtilis
J. Bacteriol.
190
5663-5671
2008
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680504
Chen
Characterization of the osmopr ...
Pseudomonas syringae
J. Bacteriol.
189
6901-6912
2007
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-
1
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1
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1
1
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1
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1
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671475
Laloknam
Halotolerant cyanobacterium Ap ...
Aphanothece halophytica
Appl. Environ. Microbiol.
72
6018-6026
2006
1
1
1
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3
8
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2
1
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1
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1
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1
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1
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1
1
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674658
Mahmood
Ion specificity and ionic stre ...
Lactococcus lactis, Lactococcus lactis IL1403
J. Biol. Chem.
281
29830-29839
2006
2
1
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