Literature summary for 7.6.2.3 extracted from

Li, Z.; Szczypka, M.; Lu, Y.; Thiele, D.; Rea, P.
The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump (1996), J. Biol. Chem., 271, 6509-6517 .

Search for more literature for 7.6.2.3

Inhibitors

Inhibitors	Comment	Organism	Structure
glutathione disulfide	-	Saccharomyces cerevisiae
vanadate	-	Saccharomyces cerevisiae
verapamil	-	Saccharomyces cerevisiae
vinblastine	-	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0141	-	S-(2,4-dinitrophenyl)glutathione[side 1]	at pH 8.0 and 25°C	Saccharomyces cerevisiae
0.0865	-	ATP	at pH 8.0 and 25°C	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
vacuolar membrane	-	Saccharomyces cerevisiae	5774	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required in form of MgATP	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + glutathione-S-conjugate[side 1]	Saccharomyces cerevisiae	-	ADP + phosphate + glutathione-S-conjugate[side 2]	-	?
ATP + H2O + glutathione-S-conjugate[side 1]	Saccharomyces cerevisiae DTY165	-	ADP + phosphate + glutathione-S-conjugate[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P39109	-	-
Saccharomyces cerevisiae DTY165	P39109	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + glutathione-S-conjugate[side 1]	-	Saccharomyces cerevisiae	ADP + phosphate + glutathione-S-conjugate[side 2]	-	?
ATP + H2O + glutathione-S-conjugate[side 1]	-	Saccharomyces cerevisiae DTY165	ADP + phosphate + glutathione-S-conjugate[side 2]	-	?
ATP + H2O + S-(2,4-dinitrophenyl)glutathione[side 1]	-	Saccharomyces cerevisiae	ADP + phosphate + S-(2,4-dinitrophenyl)glutathione[side 2]	-	?
ATP + H2O + S-(2,4-dinitrophenyl)glutathione[side 1]	-	Saccharomyces cerevisiae DTY165	ADP + phosphate + S-(2,4-dinitrophenyl)glutathione[side 2]	-	?
ATP + H2O + S-(azidophenacyl)glutathione[side 1]	-	Saccharomyces cerevisiae	ADP + phosphate + S-(azidophenacyl)glutathione[side 2]	-	?
ATP + H2O + S-(azidophenacyl)glutathione[side 1]	-	Saccharomyces cerevisiae DTY165	ADP + phosphate + S-(azidophenacyl)glutathione[side 2]	-	?
ATP + H2O + S-(metolachlor)glutathione[side 1]	-	Saccharomyces cerevisiae	ADP + phosphate + S-(metolachlor)glutathione[side 2]	-	?
ATP + H2O + S-(metolachlor)glutathione[side 1]	-	Saccharomyces cerevisiae DTY165	ADP + phosphate + S-(metolachlor)glutathione[side 2]	-	?
ATP + H2O + S-(monochlorobimane)glutathione[side 1]	-	Saccharomyces cerevisiae	ADP + phosphate + S-(monochlorobimane)glutathione[side 2]	-	?
ATP + H2O + S-(monochlorobimane)glutathione[side 1]	-	Saccharomyces cerevisiae DTY165	ADP + phosphate + S-(monochlorobimane)glutathione[side 2]	-	?

Subunits

Subunits	Comment	Organism
?	x * 156200, SDS-PAGE	Saccharomyces cerevisiae
?	x * 172000, calculated from amino acid sequence	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
cadmium factor protein	-	Saccharomyces cerevisiae
glutathione S-conjugate pump	-	Saccharomyces cerevisiae
vacuolar glutathione S-conjugate transporter	-	Saccharomyces cerevisiae
Ycf1	-	Saccharomyces cerevisiae

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.02026	-	at pH 8.0 and 25°C	Saccharomyces cerevisiae	verapamil
0.0888	-	at pH 8.0 and 25°C	Saccharomyces cerevisiae	vinblastine
0.1791	-	at pH 8.0 and 25°C	Saccharomyces cerevisiae	vanadate

General Information

General Information	Comment	Organism
physiological function	the enzyme is required for increased resistance to the toxic effects of the exogenous glutathione S-conjugate precursor, 1-chloro-2,4-dinitrobenzene, as well as cadmium	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)