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Literature summary for 7.6.2.3 extracted from

  • Cool, R.; Veenstra, M.; Van Klompenburg, W.; Heyne, R.; Mueller, M.; De Vries, E.; Van Veen, H.; Konings, W.
    S-decyl-glutathione nonspecifically stimulates the ATPase activity of the nucleotide-binding domains of the human multidrug resistance-associated protein, MRP1 (ABCC1) (2002), Eur. J. Biochem., 269, 3470-3478 .
No PubMed abstract available
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Activating Compound

Activating Compound Comment Organism Structure
decyl-maltoside 1.0 mM decyl-maltoside is able to stimulate the ATPase activities of the isolated nucleotide-binding domains more than 2fold Homo sapiens
additional information vincristine (0.046 mM), vincristine (0.046 mM) plus reduced glutathione (0.5 mM), reduced glutathione (0.5 mM), oxidized glutathione (0.5 mM), probenecid (1 mM), sulfinpyranoside (1 mM), N-ethyl-maleimide-glutathione (0.1-4.0 mM), leukotriene C4 (0.1-1.6 mM), and 17beta-estradiol (0.01-0.075 mM) have no significant effect on the basal ATPase activity of the enzyme Homo sapiens
S-decylglutathione 0.2-0.25 mM S-decylglutathione is able to stimulate the ATPase activities of the isolated nucleotide-binding domains more than 2fold Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Homo sapiens
Co2+
-
 Homo sapiens
Decanol decanol causes a small, but significant and concentration-dependent (0.1-1.0 mM) inhibition of the ATPase activity Homo sapiens
EDTA complete inhibition at 2 mM Homo sapiens
Mn2+
-
 Homo sapiens
additional information N-ethylmaleimide does not significantly inhibit the ATPase activities at concentrations of up to 2 mM Homo sapiens
N-ethylmaleimide
-
 Homo sapiens
orthovanadate the ATPase activity of GST-tagged nucleotide-binding domains 1 and 2 is inhibited more than 40% by 2 mM orthovanadate Homo sapiens
Sodium azide the ATPase activity of GST-tagged nucleotide-binding domains 1 and 2 is inhibited more than 70% by 2 mM sodium azide Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 ATP GST-fused form of nucleotide-binding domain 1 of the enzyme MRP1, at pH 7.5 and 30°C Homo sapiens
0.91
-
 ATP GST-fused form of nucleotide-binding domain 2 of the enzyme MRP1, at pH 7.5 and 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 100% activity in the presence of 2 mM Mg2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P33527
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + S-decylglutathione[side 1]
-
 Homo sapiens ADP + phosphate + S-decylglutathione[side 2]
-
 ?

Synonyms

Synonyms Comment Organism
ABCC1
-
 Homo sapiens
MRP1
-
 Homo sapiens
multidrug resistance-associated protein
-
 Homo sapiens