Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
orthovanadate | complete inhibition at 4 mM | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.64 | - |
GTP | at pH 7.4 and 37°C | Streptococcus pneumoniae | |
2.83 | - |
ATP | at pH 7.4 and 37°C | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + ciprofloxacin[side 1] | - |
Streptococcus pneumoniae | ADP + phosphate + ciprofloxacin[side 2] | - |
? | |
ATP + H2O + doxorubicin[side 1] | - |
Streptococcus pneumoniae | ADP + phosphate + doxorubicin[side 2] | - |
? | |
ATP + H2O + Hoechst33342[side 1] | - |
Streptococcus pneumoniae | ADP + phosphate + Hoechst33342[side 2] | - |
? | |
ATP + H2O + norfloxacin[side 1] | - |
Streptococcus pneumoniae | ADP + phosphate + norfloxacin[side 2] | - |
? | |
GTP + H2O + ciprofloxacin[side 1] | the enzyme has much higher GTPase activity as compared to its ATPase activity | Streptococcus pneumoniae | GDP + phosphate + ciprofloxacin[side 2] | - |
? | |
GTP + H2O + doxorubicin[side 1] | the enzyme has much higher GTPase activity as compared to its ATPase activity | Streptococcus pneumoniae | GDP + phosphate + doxorubicin[side 2] | - |
? | |
GTP + H2O + Hoechst33342[side 1] | the enzyme has much higher GTPase activity as compared to its ATPase activity. Hoechst 33342 is 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole | Streptococcus pneumoniae | GDP + phosphate + Hoechst33342[side 2] | - |
? | |
GTP + H2O + norfloxacin[side 1] | the enzyme has much higher GTPase activity as compared to its ATPase activity | Streptococcus pneumoniae | GDP + phosphate + norfloxacin[side 2] | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
multidrug ABC transporter | - |
Streptococcus pneumoniae |
PatA/PatB | - |
Streptococcus pneumoniae |