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Literature summary for 7.6.2.2 extracted from

  • Orelle, C.; Durmort, C.; Mathieu, K.; Duchene, B.; Aros, S.; Fenaille, F.; Andre, F.; Junot, C.; Vernet, T.; Jault, J.M.
    A multidrug ABC transporter with a taste for GTP (2018), Sci. Rep., 8, 2309 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
orthovanadate complete inhibition at 4 mM Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.64
-
GTP at pH 7.4 and 37°C Streptococcus pneumoniae
2.83
-
ATP at pH 7.4 and 37°C Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + ciprofloxacin[side 1]
-
Streptococcus pneumoniae ADP + phosphate + ciprofloxacin[side 2]
-
?
ATP + H2O + doxorubicin[side 1]
-
Streptococcus pneumoniae ADP + phosphate + doxorubicin[side 2]
-
?
ATP + H2O + Hoechst33342[side 1]
-
Streptococcus pneumoniae ADP + phosphate + Hoechst33342[side 2]
-
?
ATP + H2O + norfloxacin[side 1]
-
Streptococcus pneumoniae ADP + phosphate + norfloxacin[side 2]
-
?
GTP + H2O + ciprofloxacin[side 1] the enzyme has much higher GTPase activity as compared to its ATPase activity Streptococcus pneumoniae GDP + phosphate + ciprofloxacin[side 2]
-
?
GTP + H2O + doxorubicin[side 1] the enzyme has much higher GTPase activity as compared to its ATPase activity Streptococcus pneumoniae GDP + phosphate + doxorubicin[side 2]
-
?
GTP + H2O + Hoechst33342[side 1] the enzyme has much higher GTPase activity as compared to its ATPase activity. Hoechst 33342 is 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole Streptococcus pneumoniae GDP + phosphate + Hoechst33342[side 2]
-
?
GTP + H2O + norfloxacin[side 1] the enzyme has much higher GTPase activity as compared to its ATPase activity Streptococcus pneumoniae GDP + phosphate + norfloxacin[side 2]
-
?

Subunits

Subunits Comment Organism
heterodimer
-
Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
multidrug ABC transporter
-
Streptococcus pneumoniae
PatA/PatB
-
Streptococcus pneumoniae