Literature summary for 7.6.2.2 extracted from

Esser, L.; Zhou, F.; Pluchino, K.M.; Shiloach, J.; Ma, J.; Tang, W.K.; Gutierrez, C.; Zhang, A.; Shukla, S.; Madigan, J.P.; Zhou, T.; Kwong, P.D.; Ambudkar, S.V.; Gottesman, M.M.; Xia, D.
Structures of the multidrug transporter P-glycoprotein reveal asymmetric ATP binding and the mechanism of polyspecificity (2017), J. Biol. Chem., 292, 446-461 .

Search for more literature for 7.6.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 100 mM HEPES, pH 7.5, 200 mM NaCl, 25% (w/v) PEG400	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
elacridar	-	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + xenobiotic[side 1]	Mus musculus	-	ADP + phosphate + xenobiotic[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	P21447	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + calcein[side 1]	-	Mus musculus	ADP + phosphate + calcein[side 2]	-	?
ATP + H2O + cyclosporine A[side 1]	-	Mus musculus	ADP + phosphate + cyclosporine A[side 2]	-	?
ATP + H2O + quinidine[side 1]	-	Mus musculus	ADP + phosphate + quinidine[side 2]	-	?
ATP + H2O + rhodamine 123[side 1]	-	Mus musculus	ADP + phosphate + rhodamine 123[side 2]	-	?
ATP + H2O + verapamil[side 1]	-	Mus musculus	ADP + phosphate + verapamil[side 2]	-	?
ATP + H2O + xenobiotic[side 1]	-	Mus musculus	ADP + phosphate + xenobiotic[side 2]	-	?

Subunits

Subunits	Comment	Organism
?	x * 100000, SDS-PAGE	Mus musculus

Synonyms

Synonyms	Comment	Organism
multidrug transporter	-	Mus musculus
P-glycoprotein	-	Mus musculus
P-gp	-	Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
42.5	-	melting temperature	Mus musculus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)