Activating Compound | Comment | Organism | Structure |
---|---|---|---|
actinomycin D | 1 mM increases the ATPase activity of the enzyme more than 5times | Caenorhabditis elegans | |
dipyridamole | 1 mM increases the ATPase activity of the enzyme more than 5times | Caenorhabditis elegans | |
paclitaxel | 1 mM increases the ATPase activity of the enzyme more than 5times | Caenorhabditis elegans | |
progesterone | 1 mM increases the ATPase activity of the enzyme more than 5times | Caenorhabditis elegans | |
valinomycin | 1 mM increases the ATPase activity of the enzyme more than 5times | Caenorhabditis elegans |
Cloned (Comment) | Organism |
---|---|
native protein is expressed in Pichia pastoris strain SMD1163 and Sf9 insect cells, while selenomethionine-labeled protein is expressed in Hi5 cells | Caenorhabditis elegans |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, native protein is crystallized by using 100 mM HEPES pH 6.6-7.2, 200 mM sodium malonate and 19-22% (w/v) PEG 2000-MME, at 4°C. Crystals of selenomethionine-substituted protein are obtained with 100 mM HEPES pH 6.6-7.2, 200 mM ammonium phosphate monobasic and 19-22% PEG 1500 at 4°C | Caenorhabditis elegans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
vanadate | complete inhibition at 1 mM | Caenorhabditis elegans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Caenorhabditis elegans | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | P34712 | - |
- |
Purification (Comment) | Organism |
---|---|
cobalt affinity resin column chromatography, GST column chromatography, and Superdex 200 gel filtration | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + actinomycin D/in | - |
Caenorhabditis elegans | ADP + phosphate + actinomycin D/out | - |
? | |
ATP + H2O + paclitaxel/in | - |
Caenorhabditis elegans | ADP + phosphate + paclitaxel/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P-glycoprotein | - |
Caenorhabditis elegans |
P-gp | - |
Caenorhabditis elegans |