Literature summary for 7.6.2.2 extracted from

Prasad, R.; Shah, A.; Sanwal, H.; Kapoor, K.
Alanine scanning of all cysteines and construction of a functional cysteine-less Cdr1p, a multidrug ABC transporter of Candida albicans (2012), Biochem. Biophys. Res. Commun., 417, 508-513.

Search for more literature for 7.6.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli DH5alpha cells	Candida albicans

Protein Variants

Protein Variants	Comment	Organism
C1056A	the mutation leads to increased drug susceptibility while the ATPase activity of the mutant remains unaltered	Candida albicans
C1091A	the mutation leads to increased drug susceptibility while the ATPase activity of the mutant remains unaltered	Candida albicans
C1106A	the mutation leads to increased drug susceptibility while the ATPase activity of the mutant remains unaltered	Candida albicans
C1294A	the mutation leads to increased drug susceptibility while the ATPase activity of the mutant remains unaltered	Candida albicans
C1336A	the mutation leads to increased drug susceptibility while the ATPase activity of the mutant remains unaltered	Candida albicans
additional information	a cysteine-less green fluorescent protein-tagged variant of the enzyme is non-functional while the ATPase activity of the cysteine-less enzyme-His-expressing cells is 60% of that of the cells expressing wild type enzyme	Candida albicans

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + rhodamine 6-G/in	-	Candida albicans	ADP + phosphate + rhodamine 6-G/out	-	?

Synonyms

Synonyms	Comment	Organism
Cdr1p	-	Candida albicans

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Release 2023.1 (January 2023)