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Literature summary for 7.6.2.2 extracted from

  • Szeri, F.; Ilias, A.; Pomozi, V.; Robinow, S.; Bakos, E.; Varadi, A.
    The high turnover Drosophila multidrug resistance-associated protein shares the biochemical features of its human orthologues (2009), Biochim. Biophys. Acta, 1788, 402-409.
    View publication on PubMed
Search for more literature for 7.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
gene dMRP/CG6214, expression in Spodoptera frugiperda Sf9 cells Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
benzbromarone
-
 Drosophila melanogaster
indomethacin
-
 Drosophila melanogaster
MK571 the leukotriene receptor antagonist Drosophila melanogaster
additional information the enzyme is inhibited by the transported substrates Drosophila melanogaster
probenecid
-
 Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and substrate specificities, overview Drosophila melanogaster
0.231
-
 leukotriene C4/in pH 7.0, 37°C Drosophila melanogaster
0.344
-
 beta-estradiol 17-beta-D-glucuronide/in pH 7.0, 37°C Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Drosophila melanogaster 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + xenobiotic/in Drosophila melanogaster
-
 ADP + phosphate + xenobiotic/out
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
 gene dMRP/CG6214
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity, overview Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + beta-estradiol 17-beta-D-glucuronide/in
-
 Drosophila melanogaster ADP + phosphate + beta-estradiol 17-beta-D-glucuronide/out
-
 ?
ATP + H2O + calcein/in
-
 Drosophila melanogaster ADP + phosphate + calcein/out
-
 ?
ATP + H2O + carboxydichlorofluorescein/in
-
 Drosophila melanogaster ADP + phosphate + carboxydichlorofluorescein/out
-
 ?
ATP + H2O + leukotriene C4/in high activity with Drosophila melanogaster ADP + phosphate + leukotriene C4/out
-
 ?
ATP + H2O + xenobiotic/in
-
 Drosophila melanogaster ADP + phosphate + xenobiotic/out
-
 ?
additional information the fly enzyme exhibits a unique high level basal ATPase activity that is inhibited by the transported substrates, and it is capable of transporting several human MRP substrates like beta-estradiol 17-beta-D-glucuronide, leukotriene C4, calcein, fluo3 and carboxydichlorofluorescein Drosophila melanogaster ?
-
 ?

Synonyms

Synonyms Comment Organism
DMRP
-
 Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Drosophila melanogaster