Activating Compound | Comment | Organism | Structure |
---|---|---|---|
verapamil | spin-labeled, 5fold stimulation of ATPase activity | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | ATP-driven pumping of a variety of drugs out of cells by P-glycoprotein poses a serious problem to medical therapy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Homo sapiens | |
vanadate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
verapamil | pH 7.4, 37°C, EPR spin-labeled verapamil | Homo sapiens | |
0.8 | - |
ATP | pH 7.4, 37°C, MgATP | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | recombinant P-glycoprotein, expressed in Saccharomyces cerevisiae proteoliposome | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + xenobiotic/in | Homo sapiens | ATP-driven pumping of a variety of drugs out of cells, multidrug resistance, hydrophobic model of drug transport by P-glycoprotein | ADP + phosphate + xenobiotic/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + verapamil/in | EPR spin-labeled verapamil is an excellent transport substrate, the affinity is 10fold higher than for unlabeled verapamil | Homo sapiens | ADP + phosphate + verapamil/out | - |
? | |
ATP + H2O + xenobiotic/in | ATP-driven pumping of a variety of drugs out of cells, multidrug resistance, hydrophobic model of drug transport by P-glycoprotein | Homo sapiens | ADP + phosphate + xenobiotic/out | - |
? | |
ATP + H2O + xenobiotic/in | ATP-driven pumping of a variety of drugs out of cells, hydrophobic vacuum cleaner model of drug transport by P-glycoprotein | Homo sapiens | ADP + phosphate + xenobiotic/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P-glycoprotein | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.8 | - |
verapamil | pH 7.4, 37°C, EPR spin-labeled verapamil | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |