Application | Comment | Organism |
---|---|---|
medicine | MRP1 confers resistance to a wide variety of anticancer drugs | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
wild-type and mutant MRP1, expression in Sf9 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G1433D | mutation in the ABC-signature region of MRP1, mutant has negligible ATPase and vesicular transport activity, but shows normal ATP-binding, no transition state formation in the ATPase catalytic cycle | Homo sapiens |
G771D | mutation in the ABC-signature region of MRP1, mutant has negligible ATPase and vesicular transport activity, but shows normal ATP-binding, no transition state formation in the ATPase catalytic cycle | Homo sapiens |
L1430R | mutation in the ABC-signature region of MRP1, mutant has both ATPase and vesicular transport activity | Homo sapiens |
L768R | mutation in the ABC-signature region of MRP1, mutant has both ATPase and vesicular transport activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
vanadate | vanadate-sensitive ATPase activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
x * 160000, recombinant, underglycosylated form of MRP1, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + leukotriene C4/in | Homo sapiens | natural substrate of MRP1 | ADP + phosphate + leukotriene C4/out | - |
? | |
additional information | Homo sapiens | MRP1 is a key multidrug resistance ABC transporter in tumor cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + leukotriene C4/in | natural substrate of MRP1 | Homo sapiens | ADP + phosphate + leukotriene C4/out | - |
? | |
ATP + H2O + leukotriene C4/in | natural substrate of MRP1, ATP-stimulated LTC4 uptake of Sf9 inside-out membrane vesicles, roles of the highly conserved ABC-signature regions in the two non-equivalent ABC units of MRP1, major functional role of the conserved glycine residues in both ABC units of MRP1 and formation of composite catalytically active sites involving both signature regions within the ABC domains | Homo sapiens | ADP + phosphate + leukotriene C4/out | - |
? | |
ATP + H2O + N-ethylmaleimide S-glutathione/in | roles of the highly conserved ABC-signature regions in the two non-equivalent ABC units of MRP1, major functional role of the conserved glycine residues in both ABC units of MRP1 and formation of composite catalytically active sites involving both signature regions within the ABC domains | Homo sapiens | ADP + phosphate + N-ethylmaleimide S-glutathione/out | - |
? | |
additional information | MRP1 is a key multidrug resistance ABC transporter in tumor cells | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 160000, recombinant, underglycosylated form of MRP1, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MRP1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at, ATP-stimulated LTC4 uptake of inside-out vesicles | Homo sapiens |
37 | - |
assay at, N-ethylmaleimide S-glutathione-stimulated ATPase activity | Homo sapiens |