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Literature summary for 7.6.2.11 extracted from

  • Sugiyama, Y.; Nakamura, A.; Matsumoto, M.; Kanbe, A.; Sakanaka, M.; Higashi, K.; Igarashi, K.; Katayama, T.; Suzuki, H.; Kurihara, S.
    A novel putrescine exporter SapBCDF of Escherichia coli (2016), J. Biol. Chem., 291, 26343-26351 .
No PubMed abstract available
Search for more literature for 7.6.2.11

Cloned(Commentary)

Cloned (Comment) Organism
sapABCDF operon Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information generation of several sap gene deletion mutants, phenotypes, overview. Deletion of sapBCDF significantly decreases putrescine levels in the cell culture supernatant, and the DELTAsapBCDF strain does not facilitate uptake of putrescine from the culture supernatant. Complementation of the deletion mutant with the sapBCDF genes restores putrescine levels in the culture supernatant. Putrescine concentration of the culture supernatant is not influenced by DELTAsapA but is affected by DELTAsapBCDF. The Escherichia coli DELTAsapBCDF strain does not show affected resistance to antimicrobial peptide LL-37t Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + putrescine-[polyamine-binding protein][side 1] Escherichia coli
-
 ADP + phosphate + putrescine[side 2] + [polyamine-binding protein][side 1]
-
 ?
ATP + H2O + putrescine-[polyamine-binding protein][side 1] Escherichia coli MG1655
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 ADP + phosphate + putrescine[side 2] + [polyamine-binding protein][side 1]
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli Q47622 AND P0AGH3 AND P0AGH5 AND P0AAH4 AND P0AAH8 genes sapABCDF encoding probable ABC transporter periplasmic-binding protein SapA, putrescine export system permease proteins SapB and SapC, putrescine export system ATP-binding proteins SapD and SapF
-
Escherichia coli MG1655 Q47622 AND P0AGH3 AND P0AGH5 AND P0AAH4 AND P0AAH8 genes sapABCDF encoding probable ABC transporter periplasmic-binding protein SapA, putrescine export system permease proteins SapB and SapC, putrescine export system ATP-binding proteins SapD and SapF
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture Escherichia coli can grow on putrescine as a sole carbon or nitrogen source Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + putrescine-[polyamine-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + putrescine[side 2] + [polyamine-binding protein][side 1]
-
 ?
ATP + H2O + putrescine-[polyamine-binding protein][side 1]
-
 Escherichia coli MG1655 ADP + phosphate + putrescine[side 2] + [polyamine-binding protein][side 1]
-
 ?

Synonyms

Synonyms Comment Organism
putrescine export system ATP-binding protein
-
 Escherichia coli
putrescine export system permease protein
-
 Escherichia coli
putrescine exporter
-
 Escherichia coli
SapA
-
 Escherichia coli
SapB
-
 Escherichia coli
SapBCDF
-
 Escherichia coli
SapC
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

General Information

General Information Comment Organism
evolution SapABCDF is specifically distributed within gamma-proteobacteria Escherichia coli
malfunction deletion of sapBCDF significantly decreases putrescine levels in the cell culture supernatant, and the DELTAsapBCDF strain does not facilitate uptake of putrescine from the culture supernatant. Complementation of the deletion mutant with the sapBCDF genes restores putrescine levels in the culture supernatant. Putrescine concentration of the culture supernatant is not influenced by DELTAsapA but is affected by DELTAsapBCDF. The Escherichia coli DELTAsapBCDF strain does not show affected resistance to antimicrobial peptide LL-37t Escherichia coli
metabolism the enzyme complex SapBCDF exports putrescine from Escherichia coli cells to the culture supernatant, which is the natural function of the SapBCDF proteins, while PotABCD is a spermidine transporter of the ABC transporter family that takes up putrescine with lower affinity. Mechanism of polyamine export from bacterial cells to the intestinal lumen. PuuP is a putrescine importer dependent on proton-motive force and is indispensable when Escherichia coli grows on putrescine as a sole carbon or nitrogen source. PlaP is a proton-dependent putrescine importer that is important when Escherichia coli exhibits surface motility. PotE is responsible for both excretion and uptake of putrescine. PotE is a proton-dependent putrescine importer at neutral pH, but at acidic pH PotE is a putrescine-ornithine antiporter Escherichia coli
physiological function the natural function of the SapBCDF enzyme complex is the export of putrescine, Escherichia coli can grow on putrescine as a sole carbon or nitrogen source Escherichia coli