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Literature summary for 7.6.2.11 extracted from

  • Kashiwagi, K.; Innami, A.; Zenda, R.; Tomitori, H.; Igarashi, K.
    The ATPase activity and the functional domain of PotA, a component of the sermidine-preferential uptake system in Escherichia coli (2002), J. Biol. Chem., 277, 24212-24219.
    View publication on PubMed
Search for more literature for 7.6.2.11

Protein Variants

Protein Variants Comment Organism
D172N potA subunit, inactive mutant Escherichia coli
E297D potA subunit, spermidine uptake activity is greately reduced Escherichia coli
E297K potA subunit, spermidine uptake activity is reduced Escherichia coli
E297Q potA subunit, spermidine uptake activity is greately reduced Escherichia coli
F27L potA subunit, spermidine uptake activity is reduced Escherichia coli
F47L potA subunit, spermidine uptake activity is reduced Escherichia coli
L60F potA subunit, spermidine uptake activity is reduced Escherichia coli
L76P spermidine uptake activity is reduced Escherichia coli
additional information construction of mutants truncated at the carboxylic terminal domain of the potA subunit, these mutants have reduced activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
spermidine non competitive to ATP, acting as a feedback inhibitor of the spermidine transport Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.318
-
 ATP ATPase activity of the mutant L76P potA subunit Escherichia coli
0.39
-
 ATP ATPase activity of the potA subunit Escherichia coli
0.481
-
 ATP ATPase activity of the mutant E297Q potA subunit Escherichia coli
0.633
-
 ATP ATPase activity of the mutant E297K potA subunit Escherichia coli
0.855
-
 ATP ATPase activity of the mutant E297D potA subunit Escherichia coli
1.49
-
 ATP ATPase activity of the potABC complex Escherichia coli
2
-
 ATP ATPase activity of the mutant F47L potA subunit Escherichia coli
2.6
-
 ATP ATPase activity of the mutant L60F potA subunit Escherichia coli
7
-
 ATP ATPase activity of the mutant F27L potA subunit Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + spermidine/out the spermidine-preferential system, encoded by the genes potFGHI, can trasport both spermidine and putrescine Escherichia coli ADP + phosphate + spermidine/in
-
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