Literature summary for 7.6.2.10 extracted from

Wuttge, S.; Bommer, M.; Jaeger, F.; Martins, B.M.; Jacob, S.; Licht, A.; Scheffel, F.; Dobbek, H.; Schneider, E.
Determinants of substrate specificity and biochemical properties of the sn-glycerol-3-phosphate ATP binding cassette transporter (UgpB-AEC2) of Escherichia coli (2012), Mol. Microbiol., 86, 908-920.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 T1 DE3 cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with glycerol-3-phosphate, sitting drop vapor diffusion method, using 5 mM CoCl3, 5 mM NiCl2, 5 mM CdCl2, 5 mM MgCl2, 0.1 M HEPES, pH 7.5, 12% (w/v) polyethylene glycol 3350, and 0.3% octyl-beta-D-glucopyranoside	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibited by phosphate or by the signal transducing protein PhoU or the phosphodiesterase UgpQ	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + glycerol-3-phosphate/out	Escherichia coli	-	ADP + phosphate + glycerol-3-phosphate/in	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AG80	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + glycerol-3-phosphate/out	-	Escherichia coli	ADP + phosphate + glycerol-3-phosphate/in	-	?
ATP + H2O + glycerol-3-phosphocholine/out	-	Escherichia coli	ADP + phosphate + glycerol-3-phosphocholine/in	-	?
additional information	glycerol-2-phosphate is not a substrate	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
sn-glycerol-3-phosphate ATP binding cassette transporter	-	Escherichia coli
UgpB-AEC2	-	Escherichia coli

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Release 2023.1 (January 2023)