Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by NEM | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromaffin granule | - |
Homo sapiens | 42583 | - |
intracellular | - |
Homo sapiens | 5622 | - |
additional information | the presence of aminophospholipid translocase may be restricted to organelles involved in fusion processes | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
- |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphatidylserine[side 1] | Homo sapiens | - |
ADP + phosphate + phosphatidylserine[side 2] | - |
? | |
ATP + H2O + phospholipid[side 1] | Homo sapiens | - |
ADP + phosphate + phospholipid[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | inside-out erythrocytes are prepared | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphatidylserine[side 1] | - |
Homo sapiens | ADP + phosphate + phosphatidylserine[side 2] | - |
? | |
ATP + H2O + phospholipid[side 1] | - |
Homo sapiens | ADP + phosphate + phospholipid[side 2] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminophospholipid translocase | - |
Homo sapiens |
ATP-dependent specific phospholipid flippase | - |
Homo sapiens |
phosphatidylserine translocase/ATPase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | aminophospholipid translocase and ATPase II form an acylphosphate intermediate. Fusion of chromaffin granules to the plasma membrane of chromaffin cells, mediated by Ca2+, requires Mg2+ and hydrolysable ATP | Homo sapiens |
physiological function | Ca2+-dependent binding of annexin proteins to secretory granule membranes seems to be involved in the early stage of exocytosis. Aminolipids are necessary for contact and fusion between lipid vesicles or between liposomes and chromaffin granules. Thus, phosphatidylserine (PtdS) must be present on the granule outer (cytoplasmic) monolayer. Chromaffin granules possess a mechanism to maintain PtdS orientation, comparable to the ATP-dependent aminophospholipid translocase from human erythrocytes. The translocase in granules selectively transports PtdS from the luminal to the cytoplasmic monolayer, provided the incubation medium contains ATP. As this protein shares several properties with the granule vanadate-sensitive ATPase II, this ATPase is the protein responsible for aminophospholipid translocation | Homo sapiens |