Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.6.2.1 extracted from

  • Zachowski, A.; Henry, J.P.; Devaux, P.F.
    Control of transmembrane lipid asymmetry in chromaffin granules by an ATP-dependent protein (1989), Nature, 340, 75-76 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by NEM Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
chromaffin granule
-
Homo sapiens 42583
-
intracellular
-
Homo sapiens 5622
-
additional information the presence of aminophospholipid translocase may be restricted to organelles involved in fusion processes Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + phosphatidylserine[side 1] Homo sapiens
-
ADP + phosphate + phosphatidylserine[side 2]
-
?
ATP + H2O + phospholipid[side 1] Homo sapiens
-
ADP + phosphate + phospholipid[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte inside-out erythrocytes are prepared Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phosphatidylserine[side 1]
-
Homo sapiens ADP + phosphate + phosphatidylserine[side 2]
-
?
ATP + H2O + phospholipid[side 1]
-
Homo sapiens ADP + phosphate + phospholipid[side 2]
-
?

Synonyms

Synonyms Comment Organism
aminophospholipid translocase
-
Homo sapiens
ATP-dependent specific phospholipid flippase
-
Homo sapiens
phosphatidylserine translocase/ATPase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Homo sapiens

General Information

General Information Comment Organism
additional information aminophospholipid translocase and ATPase II form an acylphosphate intermediate. Fusion of chromaffin granules to the plasma membrane of chromaffin cells, mediated by Ca2+, requires Mg2+ and hydrolysable ATP Homo sapiens
physiological function Ca2+-dependent binding of annexin proteins to secretory granule membranes seems to be involved in the early stage of exocytosis. Aminolipids are necessary for contact and fusion between lipid vesicles or between liposomes and chromaffin granules. Thus, phosphatidylserine (PtdS) must be present on the granule outer (cytoplasmic) monolayer. Chromaffin granules possess a mechanism to maintain PtdS orientation, comparable to the ATP-dependent aminophospholipid translocase from human erythrocytes. The translocase in granules selectively transports PtdS from the luminal to the cytoplasmic monolayer, provided the incubation medium contains ATP. As this protein shares several properties with the granule vanadate-sensitive ATPase II, this ATPase is the protein responsible for aminophospholipid translocation Homo sapiens