Protein Variants | Comment | Organism |
---|---|---|
D256E | the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme | Saccharomyces cerevisiae |
F213S | the mutant shows increased activity with phosphatidylcholine, phosphatidylserine, and sphingomyelin compared to the wild type enzyme | Saccharomyces cerevisiae |
I615M | the mutant shows about wild type activity | Saccharomyces cerevisiae |
I615S | the mutation significantly increases sphingomyelin and phosphatidylserine transport compared to the wild type enzyme | Saccharomyces cerevisiae |
I615T | the mutant shows about wild type activity | Saccharomyces cerevisiae |
L242S | the mutant shows reduced activity with phosphatidylcholine and wild type activity with sphingomyelin | Saccharomyces cerevisiae |
N220C | the mutant shows wild type activity with phosphatidylcholine and increased activity with sphingomyelin | Saccharomyces cerevisiae |
N220S | the mutant shows reduced activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme | Saccharomyces cerevisiae |
N220S/L242S | the mutant shows reduced activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme | Saccharomyces cerevisiae |
N220T | the mutant shows increased activity with phosphatidylcholine and increased activity with sphingomyelin compared to the wild type enzyme | Saccharomyces cerevisiae |
N550S | the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme | Saccharomyces cerevisiae |
T254A | the mutant shows reduced activity with phosphatidylcholine, increased activity with phosphatidylserine, and increased activity with sphingomyelin compared to the wild type enzyme | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Saccharomyces cerevisiae | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + glycerophospholipid[side 1] | Saccharomyces cerevisiae | - |
ADP + phosphate + glycerophospholipid[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + glycerophospholipid[side 1] | - |
Saccharomyces cerevisiae | ADP + phosphate + glycerophospholipid[side 2] | - |
? | |
ATP + H2O + phosphatidylcholine[side 1] | - |
Saccharomyces cerevisiae | ADP + phosphate + phosphatidylcholine[side 2] | - |
? | |
ATP + H2O + phosphatidylethanolamine[side 1] | - |
Saccharomyces cerevisiae | ADP + phosphate + phosphatidylethanolamine[side 2] | - |
? | |
ATP + H2O + phosphatidylserine[side 1] | very low activity | Saccharomyces cerevisiae | ADP + phosphate + phosphatidylserine[side 2] | - |
? | |
ATP + H2O + sphingomyelin[side 1] | low activity | Saccharomyces cerevisiae | ADP + phosphate + sphingomyelin[side 2] | - |
? | |
additional information | no activity with phosphatidic acid | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dnf1 | - |
Saccharomyces cerevisiae |
phospholipid flippase | - |
Saccharomyces cerevisiae |
sphingomyelin flippase | - |
Saccharomyces cerevisiae |