Literature summary for 7.6.2.1 extracted from

Lopez-Marques, R.L.; Poulsen, L.R.; Palmgren, M.G.
A putative plant aminophospholipid flippase, the Arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a beta-subunit (2012), PLoS ONE, 7, e33042.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Saccharomyces cerevisiae strain w303-1a	Arabidopsis thaliana
heterologous expression of ALA1 in a enzyme-deficient Ddrs2Ddnf1Ddnf2 Saccharomyces cerevisiae mutant strain. Transient expression of N-terminally GFP-tagged ALA1 in tobacco leaves in the presence of untagged versions of three different ALIS proteins ALIS1, ALIS3, ALIS5, co-expression with any ALIS protein results in localization of GFP-ALA1 in membranes resembling either the plasma membrane or the tonoplast. Expression of GFP-tagged ALA1 lacking its beta-subunit localizes to the endoplasmic reticulum in tobacco epidermal cells	Arabidopsis thaliana

Cloned (Comment)

Organism

expressed in Saccharomyces cerevisiae strain w303-1a

Arabidopsis thaliana

heterologous expression of ALA1 in a enzyme-deficient Ddrs2Ddnf1Ddnf2 Saccharomyces cerevisiae mutant strain. Transient expression of N-terminally GFP-tagged ALA1 in tobacco leaves in the presence of untagged versions of three different ALIS proteins ALIS1, ALIS3, ALIS5, co-expression with any ALIS protein results in localization of GFP-ALA1 in membranes resembling either the plasma membrane or the tonoplast. Expression of GFP-tagged ALA1 lacking its beta-subunit localizes to the endoplasmic reticulum in tobacco epidermal cells

Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	ALA1 is targeted to the plasma membrane and following association in the endoplasmic reticulum with an ALIS protein beta-subunit	Arabidopsis thaliana	5886	-
plasma membrane	the enzyme localizes to the plant plasma membrane and has a strict requirement for an ALIS protein beta-subunit to exit the endoplasmic reticulum	Arabidopsis thaliana	5886	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-
Arabidopsis thaliana	P98204	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Synonyms

Synonyms	Comment	Organism
ALA1	-	Arabidopsis thaliana
aminophospholipid flippase	-	Arabidopsis thaliana
P4 ATPase ALA1	-	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
ATP	dependent on	Arabidopsis thaliana

General Information

General Information	Comment	Organism
evolution	ATP-dependent aminophospholipid translocases constitute a subfamily, P4 ATPases, in the superfamily of P-type ATPase pumps	Arabidopsis thaliana
physiological function	P4 ATPases are involved in establishing lipid asymmetry across plasma membranes. Asymmetric lipid distributions with aminophospholipids concentrated in the inner leaflet and sphingolipids in the outer leaflet, amongst several proposed functions, might be a prerequisite for endocytosis	Arabidopsis thaliana