Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.6.2.1 extracted from

  • Eckford, P.D.; Sharom, F.J.
    The reconstituted Escherichia coli MsbA protein displays lipid flippase activity (2010), Biochem. J., 429, 195-203.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ATP flippase activity is essentially supported only by ATP. If the ATP concentration in the assay falls below 5 mM, there is a significant decrease in flippase activity. Maximal translocation occurs at 10 mM ATP Escherichia coli
additional information GTP, AMP and AMP-PNP yield low levels of translocation compared to ATP Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
AlFx trapping of MsbA with 0.8 mM AlFx results in incomplete inhibition of activity Escherichia coli
BeFx trapping of MsbA with 0.8 mM BeFx results in incomplete inhibition of activity Escherichia coli
lipid A lipid A inhibits translocation by approx. 30% at 0.01-0.04 mg/ml Escherichia coli
additional information neither lipopolysaccharide from the Ra mutant of Escherichia coli nor deep rough chemotype lipopolysaccharide affect MsbA flippase activity significantly Escherichia coli
phosphate analogue Vi the presence of 0.1-0.2 m M Vi inhibits flippase activity of MsbA by approx. 50% Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
2 * 65000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P60752
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Lipid A/in
-
Escherichia coli ADP + phosphate + Lipid A/out
-
?
ATP + H2O + phosphatidylethanolamine/in
-
Escherichia coli ADP + phosphate + phosphatidylethanolamine/out
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 65000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
lipid flippase
-
Escherichia coli
MsbA
-
Escherichia coli