Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | flippase activity is essentially supported only by ATP. If the ATP concentration in the assay falls below 5 mM, there is a significant decrease in flippase activity. Maximal translocation occurs at 10 mM ATP | Escherichia coli | |
additional information | GTP, AMP and AMP-PNP yield low levels of translocation compared to ATP | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AlFx | trapping of MsbA with 0.8 mM AlFx results in incomplete inhibition of activity | Escherichia coli | |
BeFx | trapping of MsbA with 0.8 mM BeFx results in incomplete inhibition of activity | Escherichia coli | |
lipid A | lipid A inhibits translocation by approx. 30% at 0.01-0.04 mg/ml | Escherichia coli | |
additional information | neither lipopolysaccharide from the Ra mutant of Escherichia coli nor deep rough chemotype lipopolysaccharide affect MsbA flippase activity significantly | Escherichia coli | |
phosphate analogue Vi | the presence of 0.1-0.2 m M Vi inhibits flippase activity of MsbA by approx. 50% | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
2 * 65000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P60752 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Lipid A/in | - |
Escherichia coli | ADP + phosphate + Lipid A/out | - |
? | |
ATP + H2O + phosphatidylethanolamine/in | - |
Escherichia coli | ADP + phosphate + phosphatidylethanolamine/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 65000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
lipid flippase | - |
Escherichia coli |
MsbA | - |
Escherichia coli |