Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged Drs2p and HA-tagged Drf1p wild-type and mutant enzymes in Saccharomyces cerevisiae, subcloning in Escherichia coli strains XL1-Blue and DH5alpha | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of yeast knockout strains lacking Drs2p and/or Drf1p activity, the pan1-20 temperature-sensitive mutant is constitutively defective for Ub-dependent endocytosis but is not defective for NPFXD-dependent endocytosis at the permissive growth temperature. To sustain viability of pan1-20, Drs2p must be endocytosed through the NPFXD/Sla1p pathway | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | Drs2p, which has multiple endocytosis signals, including two NPFXDs near the C terminus and PEST-like sequences near the N terminus that may mediate ubiquitin-dependent endocytosis, localizes to the trans-Golgi network in wild-type cells and accumulates on the plasma membrane when both the Ub- and NPFXD-dependent endocytic mechanisms are inactivated, the C-terminal and N-terminal NPFXD motifs ar functionally required by Drs2p and Drf1p, overview | Saccharomyces cerevisiae | 5794 | - |
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
additional information | P4-ATPases Drs2p and Dnf1p cycle between the exocytic and endocytic pathways, and maintain a steady-state localization to internal organelles requiring endocytosis | Saccharomyces cerevisiae | - |
- |
plasma membrane | internalization of Dnf1p from the plasma membrane uses an NPFXD endocytosis signal and its recognition by Sla1p, part of an endocytic coat/adaptor complex with clathrin, Pan1p, Sla2p/End4p, and End3p | Saccharomyces cerevisiae | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phospholipid/in | Saccharomyces cerevisiae | P4-ATPases Drs2p and Dnf1p cycle between the exocytic and endocytic pathways, and maintain a steady-state localization to internal organelles requiring endocytosis signals, Drs2p is an essential endocytic cargo in cells compromised for Ub-dependent endocytosis | ADP + phosphate + phospholipid/out | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
diverse strains, overview | - |
Purification (Comment) | Organism |
---|---|
native enzymes partially by subcellular fractionation | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phospholipid/in | P4-ATPases Drs2p and Dnf1p cycle between the exocytic and endocytic pathways, and maintain a steady-state localization to internal organelles requiring endocytosis signals, Drs2p is an essential endocytic cargo in cells compromised for Ub-dependent endocytosis | Saccharomyces cerevisiae | ADP + phosphate + phospholipid/out | - |
? | |
ATP + H2O + phospholipid/in | ATP-dependent transport of phospholipids from outer to the inner monolayer of the cell membrane to maintain lipid asymmetry, the C-terminal and N-terminal NPFXD motifs ar functionally required by Drs2p and Drf1p, respectively, overview | Saccharomyces cerevisiae | ADP + phosphate + phospholipid/out | - |
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Subunits | Comment | Organism |
---|---|---|
More | the C-tail is essential for Drs2p function in protein transport bearing the functionally required NPFXD motifs, Drs2p and Drf1p both contain an N-terminally located NPFXD motif, located in the cytoplasmic tail in Drf1p, importnat for activity, overview | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Dnf1p | - |
Saccharomyces cerevisiae |
Drs2p | - |
Saccharomyces cerevisiae |
flippase | - |
Saccharomyces cerevisiae |
More | the enzymes belong to the P4-ATPase family | Saccharomyces cerevisiae |
phospholipid translocase | - |
Saccharomyces cerevisiae |