KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
additional information | - |
additional information | kinetics of redistribution of exogenously added lipids between the aqueous solution and the GUV membranes, rapid flip-flop leading to relaxation of the monolayer area difference, theoretical analysis of kinetics of shape changes provides self-consistent determination of the flip-flop rate and further kinetic parameters, overview | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Saccharomyces cerevisiae | 5783 | - |
microsome | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phospholipid/in | Saccharomyces cerevisiae | transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases | ADP + phosphate + phospholipid/out | - |
ir | |
ATP + H2O + phospholipid/in | Saccharomyces cerevisiae sec61 | transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases | ADP + phosphate + phospholipid/out | - |
ir | |
phospholipid/in | Saccharomyces cerevisiae | transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases | phospholipid/out | - |
ir | |
phospholipid/in | Saccharomyces cerevisiae sec61 | transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases | phospholipid/out | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae sec61 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially by microsome preparation | Saccharomyces cerevisiae |
Renatured (Comment) | Organism |
---|---|
preparation of proteoliposomes for enzyme reconstitution using microsome and liposomes, overview | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
flippase activity detected with unlabeled lipids by shape changes of giant unilamellar vesicles | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phospholipid/in | - |
Saccharomyces cerevisiae | ADP + phosphate + phospholipid/out | - |
ir | |
ATP + H2O + phospholipid/in | transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases | Saccharomyces cerevisiae | ADP + phosphate + phospholipid/out | - |
ir | |
ATP + H2O + phospholipid/in | - |
Saccharomyces cerevisiae sec61 | ADP + phosphate + phospholipid/out | - |
ir | |
ATP + H2O + phospholipid/in | transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases | Saccharomyces cerevisiae sec61 | ADP + phosphate + phospholipid/out | - |
ir | |
additional information | shape change of giant unilamellar vesicles can be used as a tool to study the occurrence and time scale of flippase-mediated transbilayer movement of unlabeled phospholipids, overview | Saccharomyces cerevisiae | ? | - |
? | |
additional information | shape change of giant unilamellar vesicles can be used as a tool to study the occurrence and time scale of flippase-mediated transbilayer movement of unlabeled phospholipids, overview | Saccharomyces cerevisiae sec61 | ? | - |
? | |
phospholipid/in | - |
Saccharomyces cerevisiae | phospholipid/out | - |
ir | |
phospholipid/in | transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases | Saccharomyces cerevisiae | phospholipid/out | - |
ir | |
phospholipid/in | - |
Saccharomyces cerevisiae sec61 | phospholipid/out | - |
ir | |
phospholipid/in | transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases | Saccharomyces cerevisiae sec61 | phospholipid/out | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
energy-dependent lipid flippase | - |
Saccharomyces cerevisiae |
energy-independent lipid flippase | - |
Saccharomyces cerevisiae |