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Literature summary for 7.6.2.1 extracted from

  • Papadopulos, A.; Vehring, S.; Lopez-Montero, I.; Kutschenko, L.; Stoeckl, M.; Devaux, P.F.; Kozlov, M.; Pomorski, T.; Herrmann, A.
    Flippase activity detected with unlabeled lipids by shape changes of giant unilamellar vesicles (2007), J. Biol. Chem., 282, 15559-15568.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Saccharomyces cerevisiae
additional information
-
additional information kinetics of redistribution of exogenously added lipids between the aqueous solution and the GUV membranes, rapid flip-flop leading to relaxation of the monolayer area difference, theoretical analysis of kinetics of shape changes provides self-consistent determination of the flip-flop rate and further kinetic parameters, overview Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Saccharomyces cerevisiae 5783
-
microsome
-
Saccharomyces cerevisiae
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + phospholipid/in Saccharomyces cerevisiae transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases ADP + phosphate + phospholipid/out
-
ir
ATP + H2O + phospholipid/in Saccharomyces cerevisiae sec61 transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases ADP + phosphate + phospholipid/out
-
ir
phospholipid/in Saccharomyces cerevisiae transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases phospholipid/out
-
ir
phospholipid/in Saccharomyces cerevisiae sec61 transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases phospholipid/out
-
ir

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae sec61
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially by microsome preparation Saccharomyces cerevisiae

Renatured (Commentary)

Renatured (Comment) Organism
preparation of proteoliposomes for enzyme reconstitution using microsome and liposomes, overview Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
flippase activity detected with unlabeled lipids by shape changes of giant unilamellar vesicles Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phospholipid/in
-
Saccharomyces cerevisiae ADP + phosphate + phospholipid/out
-
ir
ATP + H2O + phospholipid/in transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases Saccharomyces cerevisiae ADP + phosphate + phospholipid/out
-
ir
ATP + H2O + phospholipid/in
-
Saccharomyces cerevisiae sec61 ADP + phosphate + phospholipid/out
-
ir
ATP + H2O + phospholipid/in transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases Saccharomyces cerevisiae sec61 ADP + phosphate + phospholipid/out
-
ir
additional information shape change of giant unilamellar vesicles can be used as a tool to study the occurrence and time scale of flippase-mediated transbilayer movement of unlabeled phospholipids, overview Saccharomyces cerevisiae ?
-
?
additional information shape change of giant unilamellar vesicles can be used as a tool to study the occurrence and time scale of flippase-mediated transbilayer movement of unlabeled phospholipids, overview Saccharomyces cerevisiae sec61 ?
-
?
phospholipid/in
-
Saccharomyces cerevisiae phospholipid/out
-
ir
phospholipid/in transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases Saccharomyces cerevisiae phospholipid/out
-
ir
phospholipid/in
-
Saccharomyces cerevisiae sec61 phospholipid/out
-
ir
phospholipid/in transbilayer movement of phospholipids in an unidirectional fashion in biological membranes is mediated by energy-dependent and energy-independent flippases Saccharomyces cerevisiae sec61 phospholipid/out
-
ir

Synonyms

Synonyms Comment Organism
energy-dependent lipid flippase
-
Saccharomyces cerevisiae
energy-independent lipid flippase
-
Saccharomyces cerevisiae