Literature summary for 7.6.2.1 extracted from

Regev, R.; Katzir, H.; Yeheskely-Hayon, D.; Eytan, G.D.
Modulation of P-glycoprotein-mediated multidrug resistance by acceleration of passive drug permeation across the plasma membrane (2007), FEBS J., 274, 6204-6214.

Activating Compound

Activating Compound	Comment	Organism	Structure
CDC50 proteins	CDC50A and CDC50B, cloning and expression of the abut 60 kDa proteins, required for full translocase activity, 2.5-5fold activition, proteins are pivotal factors in the trafficking of ATP8B1 to the plasma membrane and thus may be essential determinants of ATP8B1-related disease, physical interacion with ATP8B1, overview	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
isolated stable expression of N-terminally GFP-tagged ATP8B1 in UPS-1 cells, a nonpolarized CHO-K1 mutant cell line with a defect in the nonendocytic uptake of the NBD-PS analogue, by lentiviral transduction leading to protein localization in the endoplasmic reticulum, co-expression with N-terminally HA-tagged CDC50 proteins results in relocalization of ATP8B1 from the endoplasmic reticulum to the plasma membrane, natural phosphatidylserine exposure in the outer leaflet of the plasma membrane was reduced by 17%-25% in cells coexpressing ATP8B1 and CDC50 proteins in comparison with cells expressing ATP8B1 alone, coexpression of ATP8B1 and CDC50A in WIF-B9 cells resulted in colocalization of both proteins in the canalicular membrane, overview	Homo sapiens

Cloned (Comment)

Organism

isolated stable expression of N-terminally GFP-tagged ATP8B1 in UPS-1 cells, a nonpolarized CHO-K1 mutant cell line with a defect in the nonendocytic uptake of the NBD-PS analogue, by lentiviral transduction leading to protein localization in the endoplasmic reticulum, co-expression with N-terminally HA-tagged CDC50 proteins results in relocalization of ATP8B1 from the endoplasmic reticulum to the plasma membrane, natural phosphatidylserine exposure in the outer leaflet of the plasma membrane was reduced by 17%-25% in cells coexpressing ATP8B1 and CDC50 proteins in comparison with cells expressing ATP8B1 alone, coexpression of ATP8B1 and CDC50A in WIF-B9 cells resulted in colocalization of both proteins in the canalicular membrane, overview

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	mutations in isozyme ATP8B1 cause progressive familial intrahepatic cholestasis type 1 and benign recurrent intrahepatic cholestasis type 1, overview	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum membrane	ATP8B1 in absence of CDC50A and CDC50	Homo sapiens	5789	-
plasma membrane	CDC50A and CDC50B are pivotal factors in the trafficking of ATP8B1 to the plasma membrane and thus may be essential determinants of ATP8B1-related disease	Homo sapiens	5886	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + phosphatidylserine/in	Homo sapiens	selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the plasma membrane, proteins are pivotal factors in the trafficking of ATP8B1 to the plasma membrane and thus may be essential determinants of ATP8B1-related disease	ADP + phosphate + phosphatidylserine/out	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O43520	mRNA of ATP8B1; isozyme ATP8B1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + H2O + 1-palmitoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-sn-glycero-3-phospho-L-serine/in	-	Homo sapiens	ADP + phosphate + 1-palmitoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-sn-glycero-3-phospho-L-serine/out	-	?
ATP + H2O + phosphatidylserine/in	selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the plasma membrane, proteins are pivotal factors in the trafficking of ATP8B1 to the plasma membrane and thus may be essential determinants of ATP8B1-related disease	Homo sapiens	ADP + phosphate + phosphatidylserine/out	-	?
ATP + H2O + phosphatidylserine/in	selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the plasma membrane	Homo sapiens	ADP + phosphate + phosphatidylserine/out	-	?
additional information	recombinant ATP8B1 Physically Interacts with HA-CDC50A and HA-CDC50B, uptake of 7-nitro-2-1,3-benzoxadiazol-4-yllabeled phosphatidylcholine, phosphatidylethanolamine and sphingomyelin is not dependent on ATP8B1-CDC50 expression	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
aminophospholipid translocase	-	Homo sapiens
ATP8A1	-	Homo sapiens
More	the enzyme belongs to the P-type ATPases	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens