Literature summary for 7.6.2.1 extracted from

Liu, J.; Epand, R.F.; Durrant, D.; Grossman, D.; Chi, N.W.; Epand, R.M.; Lee, R.M.
Role of phospholipid scramblase 3 in the regulation of tumor necrosis factor-alpha-induced apoptosis (2008), Biochemistry, 47, 4518-4529.

Search for more literature for 7.6.2.1

Activating Compound

Activating Compound	Comment	Organism	Structure
tBid	PLS3 and tBid may form a bidirectional positive feedback loop that is antagonized by Bcl-2	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
F258V	site-directed mutagenesis, mutation of the calcium binding motif abolishes the lipid flip-flop activity of PLS3, a dominant negative mutant of PLS3, expression of the PLS3(F258V) mutant in HEK-293 cells results in a phenotype still consistent with low PLS3 activity despite the presence of normal PLS3 in these cells due to spontaneous rate of flip-flop	Homo sapiens
additional information	downregulation of PLS3 in HeLa cells by siRNA	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Bcl-2	PLS3 and tBid may form a bidirectional positive feedback loop that is antagonized by Bcl-2	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrial membrane	-	Homo sapiens	31966	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	phospholipid scramblase 3 is involved in the regulation of tumor necrosis factor-alpha-induced apoptosis, overexpression of PLS3 enhances, whereas downregulation of PLS3 delays, TNF-R-induced apoptosis and targeting of tBid to mitochondria, as well as overexpression increases and downregulation decreases the percentage of cardiolipin on the mitochondrial surface and tBid binding capacity, overview. PLS3 is an important downstream effector of Bcl-2 and tBid in apoptosis,PLS3 regulation, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HeLa cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-lauroyl-2-(1'-pyrenebutyroyl)-sn-glycero-3-phosphocholine/in	i.e. pyrene-10-PC, development of a transbilayer lipid flip-flop assay in PLS3 proteoliposomes, overview	Homo sapiens	1-lauroyl-2-(1'-pyrenebutyroyl)-sn-glycero-3-phosphocholine/out	-	r
additional information	phospholipid scramblase 3 is involved in the regulation of tumor necrosis factor-alpha-induced apoptosis, overexpression of PLS3 enhances, whereas downregulation of PLS3 delays, TNF-R-induced apoptosis and targeting of tBid to mitochondria, as well as overexpression increases and downregulation decreases the percentage of cardiolipin on the mitochondrial surface and tBid binding capacity, overview. PLS3 is an important downstream effector of Bcl-2 and tBid in apoptosis,PLS3 regulation, overview	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
phospholipid scramblase 3	-	Homo sapiens
PLS3	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)