Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Kdo2-lipid A | - |
Escherichia coli | |
Phospholipids | The activity of purified MsbA is dependent upon the presence of phospholipids. | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
the gene MsbA is cloned into pET28b behind the T7 promoter in-frame with an N-terminal His6tag | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A270T | mutant and wild-type enzymes have similar activities at 30°C, but the mutant activity is decreased significant at 42°C | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
vanadate | maximal half-inhibition at 0.035 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.878 | - |
ATP | pH 7.5, 37°C, KM value of purified, detergent-solubilized MsbA, whereas the KM value calculated in the presence of 0.05 mM KdO2-lipid A is decreased by more than half | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | 0.004 | activity of purified, detergent-solubilized MsbA | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Escherichia coli |