Literature summary for 7.5.2.5 extracted from

Bos, M.P.; Tommassen, J.
The LptD chaperone LptE is not directly involved in lipopolysaccharide transport in Neisseria meningitidis (2011), J. Biol. Chem., 286, 28688-28696 .

Search for more literature for 7.5.2.5

Cloned(Commentary)

Cloned (Comment)	Organism
gene lptB, genetic organization, overview	Neisseria meningitidis serogroup B / serotype 15

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a several HB-1DELTAlptB lptB deletion mutants, phenotypes, overview. The expression of PagL in the lptH, lptC, lptF, and lptG mutants does not affect LPS mobility, demonstrating that the residual LPS present in these strains does not reach the cell surface. PagL is an outer membrane (OM) enzyme that can remove an acyl chain from LPS when LPS is present at its proper location, i.e. the outer leaflet of the OM	Neisseria meningitidis serogroup B / serotype 15

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
inner membrane	the inner membrane proteins LptB, LptC, LptF, and LptG form a complex	Neisseria meningitidis serogroup B / serotype 15	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Neisseria meningitidis serogroup B / serotype 15

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + lipopolysaccharide[side 1]	Neisseria meningitidis serogroup B / serotype 15	-	ADP + phosphate + lipopolysaccharide[side 2]	-	?
ATP + H2O + lipopolysaccharide[side 1]	Neisseria meningitidis serogroup B / serotype 15 H44/76	-	ADP + phosphate + lipopolysaccharide[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Neisseria meningitidis serogroup B / serotype 15	E6MYT4	and strain HB1, derived from H44/76 with the capsule locus, including the galE gene, inactivated resulting in expression of truncated LPS	-
Neisseria meningitidis serogroup B / serotype 15 H44/76	E6MYT4	and strain HB1, derived from H44/76 with the capsule locus, including the galE gene, inactivated resulting in expression of truncated LPS	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + lipopolysaccharide[side 1]	-	Neisseria meningitidis serogroup B / serotype 15	ADP + phosphate + lipopolysaccharide[side 2]	-	?
ATP + H2O + lipopolysaccharide[side 1]	-	Neisseria meningitidis serogroup B / serotype 15 H44/76	ADP + phosphate + lipopolysaccharide[side 2]	-	?

Synonyms

Synonyms	Comment	Organism
lptB	-	Neisseria meningitidis serogroup B / serotype 15

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Neisseria meningitidis serogroup B / serotype 15

General Information

General Information	Comment	Organism
evolution	the Lpt system does not function in a completely conserved manner in all Gram-negative bacteria	Neisseria meningitidis serogroup B / serotype 15
malfunction	the phenotypes of Neisseria meningitidis strains lacking LptB, LptC, LptH (EC 2.7.8.43), LptF, and LptG are identical to those lacking LptD or MsbA, i.e. the knockout mutants are viable but leaky and produce only very little LPS, which is not present at the cell surface	Neisseria meningitidis serogroup B / serotype 15
physiological function	biosynthesis of lipopolysaccharide (LPS) in Gram-negative bacteria requires the transport of LPS to its destination, the outer leaflet of the outer membrane. In contrast to Escherichia coli, Neisseria meningitidis can survive without LPS and tolerates inactivation of genes involved in LPS synthesis and transport. LptA, LptB, LptC, LptE, LptF, and LptG proteins are not essential in Neisseria meningitidis and are not required for an essential process such as phospholipid transport. The LptD chaperone LptE is not directly involved in lipopolysaccharide transport in Neisseria meningitidis. LptC binds LPS	Neisseria meningitidis serogroup B / serotype 15

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Release 2023.1 (January 2023)