Literature summary for 7.5.2.5 extracted from

Zhang, C.; Chen, X.; Stephanopoulos, G.; Too, H.P.
Efflux transporter engineering markedly improves amorphadiene production in Escherichia coli (2016), Biotechnol. Bioeng., 113, 1755-1763 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene lptB, the gene belongs to the lipopolysaccharide transport system encoded by operon lptCABFG, overexpression of the lptCABFG operon in Escherichia coli strain K12 MG1655 DELTArecADELTAendA DE3, co-overexpression of LptCABFG and LptD or LptD and TolC, all genes and operons are inserted into modified RK2 (amp) plasmid with the Pm promoter replaced with TM1 promoter, quantitative real-time PCR expression analysis	Escherichia coli

Cloned (Comment)

Organism

gene lptB, the gene belongs to the lipopolysaccharide transport system encoded by operon lptCABFG, overexpression of the lptCABFG operon in Escherichia coli strain K12 MG1655 DELTArecADELTAendA DE3, co-overexpression of LptCABFG and LptD or LptD and TolC, all genes and operons are inserted into modified RK2 (amp) plasmid with the Pm promoter replaced with TM1 promoter, quantitative real-time PCR expression analysis

Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	amorphadiene, the precursor of antimalarial drug artemisinin from Artemisia annua, is secreted from Escherichia coli cells overexpressing the biosynthetic pathway. The overexpression of transporters in the lipopolysaccharide transport system (msbA, lptD, lptCABFG) improves amorphadiene (AD) production. AD production in both early stage (8 h) and final stage (24 h) is increased by more than twofold in the strains that overexpress lptCABFG or msbA. But co-overexpression of LptCABFG and LptD or LptD and TolC does not enhance AD-specific production synergistically, despite the fact that the AD titer is increased mainly due to the increased cell density, overview	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
inner membrane	-	Escherichia coli	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + lipopolysaccharide[side 1]	Escherichia coli	-	ADP + phosphate + lipopolysaccharide[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A9V1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + lipopolysaccharide[side 1]	-	Escherichia coli	ADP + phosphate + lipopolysaccharide[side 2]	-	?

Synonyms

Synonyms	Comment	Organism
lptB	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	the enzme is an LPS transporters. LPS transporters are ABC exporters that are known to export extremely hydrophobic compounds, such as lipids, drugs, and steroids. LptB is implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli	Escherichia coli