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Literature summary for 7.5.2.1 extracted from

  • Bao, H.; Dalal, K.; Cytrynbaum, E.; Duong, F.
    Sequential action of MalE and maltose allows coupling ATP hydrolysis to translocation in the MalFGK2 transporter (2015), J. Biol. Chem., 290, 25452-25460 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.5.2.1

Activating Compound

Activating Compound Comment Organism Structure
MalE MalE and maltose together stimulate the ATPase activity of the enzyme. MalE stabilizes the enzyme in the outward-facing conformation Escherichia coli
maltose MalE and maltose together stimulate the ATPase activity of the enzyme. Maltose triggers return to the inward-facing state for substrate and Pi release Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.232
-
 ATP at pH 7.5 and 37°C Escherichia coli
0.274
-
 ATP in the presence of MalE, at pH 7.5 and 37°C Escherichia coli
0.283
-
 ATP in the presence of MalE and maltose, at pH 7.5 and 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + maltose-[maltose-binding protein][side 1] Escherichia coli
-
 ADP + phosphate + maltose[side 2] + [maltose-binding protein][side 1]
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + maltose-[maltose-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + maltose[side 2] + [maltose-binding protein][side 1]
-
 ?

Synonyms

Synonyms Comment Organism
MalFGK2
-
 Escherichia coli
maltose ABC transporter
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 ATP at pH 7.5 and 37°C Escherichia coli
1.2
-
 ATP in the presence of MalE, at pH 7.5 and 37°C Escherichia coli
7.8
-
 ATP in the presence of MalE and maltose, at pH 7.5 and 37°C Escherichia coli