Protein Variants | Comment | Organism |
---|---|---|
D380G | substitution of aspartate for glycine in the maltose-binding site of MalF likely generates a futile cycle by preventing maltose from binding to MalFGK2 during the catalytic cycle | Escherichia coli |
E159Q | the transporter containing the MalK-E159Q mutation is defective in ATP hydrolysis | Escherichia coli |
additional information | in deletion mutant MalG-DELTAscoop a four-residue deletion of a periplasmic loop of MalG limits its reach into the maltose-binding pocket of MBP, allowing maltose to remain associated with MBP during the catalytic cycle. Uncoupling of transport and ATP hydrolysis in the MalG-DELTAscoop and MalF-G380D mutants, overview | Escherichia coli |
W230C | site-directed mutagenesis, mutation in the maltose binding protein, the mutant shows altered kinetics of substrate binding compared to the wild-type maltose binding protein | Escherichia coli |
W340C | site-directed mutagenesis, mutation in the maltose binding protein, the mutant shows altered kinetics of substrate binding compared to the wild-type maltose binding protein | Escherichia coli |
W62C | site-directed mutagenesis, mutation in the maltose binding protein, the mutant shows altered kinetics of substrate binding compared to the wild-type maltose binding protein, but retains good function in maltose transport and MBP-stimulated ATPase activities | Escherichia coli |
Y155C | site-directed mutagenesis, mutation in the maltose binding protein, the mutant shows altered kinetics of substrate binding compared to the wild-type maltose binding protein | Escherichia coli |
Y341C | site-directed mutagenesis, mutation in the maltose binding protein, the mutant shows altered kinetics of substrate binding compared to the wild-type maltose binding protein, but retaines good function in maltose transport and MBP-stimulated ATPase activities | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + maltose/out | Escherichia coli | - |
ADP + phosphate + maltose/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + maltose/out | - |
Escherichia coli | ADP + phosphate + maltose/in | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | the maltose transporter consists of a periplasmic maltose binding protein and a multisubunit membrane transporter, MalFGK2 | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
MalFGK2 | - |
Escherichia coli |
maltose transporter | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
maltose transport assay | Escherichia coli |
8 | - |
ATPase assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the ATP-binding cassette superfamily | Escherichia coli |
malfunction | the addition of ATP and EDTA (to trigger the outwardfacing conformation) to the MBP-MalFGK2 system in the absence of maltose increases the mobility of W62C-SL in the MalG-DELTAscoop system but decreases it in the wild-type | Escherichia coli |
physiological function | the enzyme couples the energy from ATP hydrolysis to the active transport of substrates across the membrane | Escherichia coli |