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Literature summary for 7.5.2.1 extracted from

  • Khare, D.; Oldham, M.L.; Orelle, C.; Davidson, A.L.; Chen, J.
    Alternating access in maltose transporter mediated by rigid-body rotations (2009), Mol. Cell, 33, 528-536.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain AD110 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
purified native MalFGK2 and and selenium methionine-labeled MalFGK2, in 20 mM Tris, pH 8.0, 50 mM NaCl, and 0.03% n-undecyl-beta-D-maltopyranoside, addtion of 5 mM 2-mercaptoethanol and 5 mM DTT for the SeMet-enzyme. Mixing of 1 mg/ml protein and reservoir solution, containing 11.5% PEG 4000, 0.1M N-(2-acetamido)iminodiacetic acid, pH 6.5, 0.1 M NaCl, and 0.1 M Li2SO4, in a 2:1 ratio, at 20°C in sitting drops, crystals appear after 24 h and grow to full size within 1 week, X-ray diffraction structure determination and anaylsis at 2.8 A resolution Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Escherichia coli 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + maltose/out Escherichia coli
-
ADP + phosphate + maltose/in
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P68187
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant MalFGK2 from Escherichia coli strain AD110 by cobalt affinity chromatography and gel filtration Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
recombinant wild-type and mutant purified MalFGK2 are reconstituted into proteoliposomes at a lipid-to-protein ratio of 100:1 in g/ml Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + maltose/out
-
Escherichia coli ADP + phosphate + maltose/in
-
?

Subunits

Subunits Comment Organism
More maltose transporter in the inward-facing conformation with rotation axes iand in outward-facing structure, overview Escherichia coli

Synonyms

Synonyms Comment Organism
maltose transporter MalFGK2
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at, enzyme reconstituted in proteoliposomes Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at, enzyme reconstituted in proteoliposomes Escherichia coli