Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain AD110 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified native MalFGK2 and and selenium methionine-labeled MalFGK2, in 20 mM Tris, pH 8.0, 50 mM NaCl, and 0.03% n-undecyl-beta-D-maltopyranoside, addtion of 5 mM 2-mercaptoethanol and 5 mM DTT for the SeMet-enzyme. Mixing of 1 mg/ml protein and reservoir solution, containing 11.5% PEG 4000, 0.1M N-(2-acetamido)iminodiacetic acid, pH 6.5, 0.1 M NaCl, and 0.1 M Li2SO4, in a 2:1 ratio, at 20°C in sitting drops, crystals appear after 24 h and grow to full size within 1 week, X-ray diffraction structure determination and anaylsis at 2.8 A resolution | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + maltose/out | Escherichia coli | - |
ADP + phosphate + maltose/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P68187 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant MalFGK2 from Escherichia coli strain AD110 by cobalt affinity chromatography and gel filtration | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
recombinant wild-type and mutant purified MalFGK2 are reconstituted into proteoliposomes at a lipid-to-protein ratio of 100:1 in g/ml | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + maltose/out | - |
Escherichia coli | ADP + phosphate + maltose/in | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | maltose transporter in the inward-facing conformation with rotation axes iand in outward-facing structure, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
maltose transporter MalFGK2 | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at, enzyme reconstituted in proteoliposomes | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at, enzyme reconstituted in proteoliposomes | Escherichia coli |