Literature summary for 7.5.2.1 extracted from

Landmesser, H.; Stein, A.; Bluschke, B.; Brinkmann, M.; Hunke, S.; Schneider, E.
Large-scale purification, dissociation and functional reassembly of the maltose ATP-binding cassette transporter (MalFGK2) of Salmonella typhimurium (2002), Biochim. Biophys. Acta, 1565, 64-72.

Search for more literature for 7.5.2.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants	Comment	Organism
Q140K	normal intrinsic ATPase activity in soluble state but no hydrolysis of ATP when assembled with MalFG	Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors	Comment	Organism	Structure
enzyme IIA Glc	-	Salmonella enterica subsp. enterica serovar Typhimurium
vanadate	-	Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	ATP	-	Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + maltose/out	Salmonella enterica subsp. enterica serovar Typhimurium	translocation of maltose across biological membranes	ADP + phosphate + maltose/in	-	?

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification of recombinant subunits	Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.25	-	-	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + maltose/out	-	Salmonella enterica subsp. enterica serovar Typhimurium	ADP + phosphate + maltose/in	-	?
ATP + H2O + maltose/out	translocation of maltose across biological membranes	Salmonella enterica subsp. enterica serovar Typhimurium	ADP + phosphate + maltose/in	-	?

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Release 2023.1 (January 2023)