Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 100 mM HEPES pH 7.5, 1.0 M trisodium citrate | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
V57P/W551P | the mutant essentially shows similar peptide-binding affinity compared to the wild type enzyme | Thermotoga maritima |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Thermotoga maritima | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73 | - |
gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WXR2 | - |
- |
Thermotoga maritima ATCC 43589 | Q9WXR2 | - |
- |
Purification (Comment) | Organism |
---|---|
Sepharose column chromatography, and Superdex 200 gel filtration | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + GGGQATGPPNTSYMPGMPGD-[GGGQATGPPNTSYMPGMPGD-binding protein][side 1] | - |
Thermotoga maritima | ADP + phosphate + GGGQATGPPNTSYMPGMPGD[side 2] + [GGGQATGPPNTSYMPGMPGD-binding protein][side 1] | - |
? | |
ATP + H2O + GGGQATGPPNTSYMPGMPGD-[GGGQATGPPNTSYMPGMPGD-binding protein][side 1] | - |
Thermotoga maritima ATCC 43589 | ADP + phosphate + GGGQATGPPNTSYMPGMPGD[side 2] + [GGGQATGPPNTSYMPGMPGD-binding protein][side 1] | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 71000, SDS-PAGE | Thermotoga maritima |
monomer | 1 * 73000, calculated from amino acid sequence | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
oligopeptide ABC transporter | - |
Thermotoga maritima |
oligopeptide-binding protein | - |
Thermotoga maritima |
OppA | - |
Thermotoga maritima |