Cloned (Comment) | Organism |
---|---|
expression of Arabidopsis thaliana AtOPT6 in oocytes by swapping the 5'-untranslated region of AtOPT6 with the 5'-UTR of ScOPT1 from Saccharomyces cerevisiae | Saccharomyces cerevisiae |
optimization of AtOPT6 expression in oocytes by swapping the 5'-untranslated region of AtOPT6 with the 5'-UTR of ScOPT1 from Saccharomyces cerevisiae | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isozyme AtOPT6 | - |
Saccharomyces cerevisiae | - |
OPT1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no expression of OPTs in in the root epidermis or root hair cells | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity and current kinetics, overview | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + AtCLE19p12/out | in Xenopus laevis oocytes, injected with AtOPT6 cRNA | Arabidopsis thaliana | ADP + phosphate + AtCLE19p12/in | - |
? | |
ATP + H2O + CLE/out | in Xenopus laevis oocytes, injected with AtOPT6 cRNA, CLE is transported by AtOPT6 with high affinity | Arabidopsis thaliana | ADP + phosphate + CLE/in | - |
? | |
ATP + H2O + GGFL/out | in Xenopus laevis oocytes, injected with AtOPT6 cRNA. GGFL-induced currents are pH-dependent | Arabidopsis thaliana | ADP + phosphate + GGFL/in | - |
? | |
ATP + H2O + GGFL/out | in Xenopus laevis oocytes, injected with ScOPT1 cRNA | Saccharomyces cerevisiae | ADP + phosphate + GGFL/in | - |
? | |
ATP + H2O + GGFM/out | in Xenopus laevis oocytes, injected with AtOPT6 cRNA | Arabidopsis thaliana | ADP + phosphate + GGFM/in | - |
? | |
ATP + H2O + GSH/out | in Xenopus laevis oocytes, injected with AtOPT6 cRNA. GSH-induced currents are pH-dependent, low affinity GSH transport of AtOPT6 | Arabidopsis thaliana | ADP + phosphate + GSH/in | - |
? | |
ATP + H2O + GSH/out | in Xenopus laevis oocytes, injected with ScOPT1 cRNA | Saccharomyces cerevisiae | ADP + phosphate + GSH/in | - |
? | |
ATP + H2O + GSSG/out | in Xenopus laevis oocytes, injected with ScOPT1 cRNA | Saccharomyces cerevisiae | ADP + phosphate + GSSG/in | - |
? | |
ATP + H2O + KLG/out | in Xenopus laevis oocytes, injected with AtOPT6 cRNA | Arabidopsis thaliana | ADP + phosphate + KLG/in | - |
? | |
ATP + H2O + KLGL/out | in Xenopus laevis oocytes, injected with AtOPT6 cRNA | Arabidopsis thaliana | ADP + phosphate + KLGL/in | - |
? | |
ATP + H2O + KLLLG/out | in Xenopus laevis oocytes, injected with AtOPT6 cRNA | Arabidopsis thaliana | ADP + phosphate + KLLLG/in | - |
? | |
ATP + H2O + KLLLLG/out | in Xenopus laevis oocytes, injected with AtOPT6 cRNA | Arabidopsis thaliana | ADP + phosphate + KLLLLG/in | - |
? | |
ATP + H2O + phytochelatin/out | i.e. (gamma -Glu-Cys) 2 -Gly, in Xenopus laevis oocytes, injected with AtOPT6 cRNA | Arabidopsis thaliana | ADP + phosphate + phytochelatin/in | - |
? | |
ATP + H2O + phytochelatin/out | i.e. (gamma -Glu-Cys) 2 -Gly, in Xenopus laevis oocytes, injected with ScOPT1 cRNA | Saccharomyces cerevisiae | ADP + phosphate + phytochelatin/in | - |
? | |
additional information | AtOPT6 displays high affinity for penta- and dodecapeptides, but low affinity for GSH, substrate specificity of AtOPT6, inward currents caused by KLLG and KLLLG application are 3 to 7fold greater than with KLG, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | ScOPT1 substrate specificity, overview. KLLLG, RPPGF, DRVYIHPF or AtCLE19p12 cause no inward currents in ScOPT1-expressing oocytes | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtOPT6 | - |
Arabidopsis thaliana |
glutathione transporter | - |
Saccharomyces cerevisiae |
oligopeptide transporter | - |
Saccharomyces cerevisiae |
oligopeptide transporter | - |
Arabidopsis thaliana |
OPT6 | - |
Arabidopsis thaliana |
ScOPT1 | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | ScOPT1 is a high affinity GSH transporter. Fungal OPTs function in nutrient uptake from the environment | Saccharomyces cerevisiae |
physiological function | unlike in fungi, plant OPTs do not function in nutrient uptake from the environment, but OPT6 can transport plant signaling peptides | Arabidopsis thaliana |