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Literature summary for 7.4.2.6 extracted from

  • Balan, A.; Ferreira, R.C.; Ferreira, L.C.
    Production of the refolded oligopeptide-binding protein (OppA) encoded by the citrus pathogen Xanthomonas axonopodis pv. Citri (2008), Genet. Mol. Res., 7, 117-126.
    View publication on PubMed
Search for more literature for 7.4.2.6

Cloned(Commentary)

Cloned (Comment) Organism
gene oppA, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) as mainly insoluble protein Xanthomonas axonopodis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated Xanthomonas axonopodis 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + olipopeptide/out Xanthomonas axonopodis the oligopeptide-binding protein, OppA, binds and ush­ers oligopeptide substrates to the membrane-associated oligopeptide permease, Opp, a multi-component ABC-type transporter involved in the uptake of oligopeptides ADP + phosphate + oligopeptide/in
-
 ?

Organism

Organism UniProt Comment Textmining
Xanthomonas axonopodis
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 pv. citri, i.e. Xanthomonas citri, strain 306, gene oppA
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Purification (Commentary)

Purification (Comment) Organism
recombinant refolded and soluble His-tagged OppA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Xanthomonas axonopodis

Renatured (Commentary)

Renatured (Comment) Organism
solubilization and refolding of recombinant insoluble His-tagged OppA expressed in Escherichia coli strain BL21(DE3) by dilution with 100fold volumes of refolding buffer, PBS, pH 7.4, containing 1 M urea, 10% glycerol, 0.005% Tween 20, 0.5 mM PMSF and 5 mM DTT, and maintainance under vigorous agitation at 4°C for 8 h Xanthomonas axonopodis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + olipopeptide/out
-
 Xanthomonas axonopodis ADP + phosphate + oligopeptide/in
-
 ?
ATP + H2O + olipopeptide/out the oligopeptide-binding protein, OppA, binds and ush­ers oligopeptide substrates to the membrane-associated oligopeptide permease, Opp, a multi-component ABC-type transporter involved in the uptake of oligopeptides Xanthomonas axonopodis ADP + phosphate + oligopeptide/in
-
 ?

Subunits

Subunits Comment Organism
More circular dichroism spectrum of the soluble refolded recombinant OppA and secondary structure of the recombinant protein, overview Xanthomonas axonopodis

Synonyms

Synonyms Comment Organism
membrane-associated oligopeptide permease
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 Xanthomonas axonopodis
oligopeptide-binding protein
-
 Xanthomonas axonopodis
Opp
-
 Xanthomonas axonopodis
OppA
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 Xanthomonas axonopodis