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Literature summary for 7.4.2.5 extracted from
- Peptide specificity and lipid activation of the lysosomal transport complex ABCB9 (TAPL) (2008), J. Biol. Chem., 283, 17083-17091.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| TAPL containing a C-terminal His10 tag or a Strep-tag II is cloned for expression in Sf9 insect cells infected with recombinant baculovirus | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosomal membrane | - |
Homo sapiens | 5765 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
determined by SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + peptide/in | Homo sapiens | - |
ADP + phosphate + peptide/out | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| crude membranes containing TAPL are prepared from Sf9 insect cells, furthermore a SP Sepharose Fast Low column and a Zn2+-iminodiacetate column are used | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000003 | - |
peptide transport rate, TAPL reconstitution in liposomes of defined lipid composition, DOPC-eggPG, 9 to 1 | Homo sapiens |
| 0.000004 | - |
peptide transport rate, TAPL reconstitution in liposomes of defined lipid composition, DOPC-DOPE, 9 to 1 | Homo sapiens |
| 0.000006 | - |
peptide transport rate, TAPL reconstituted in liposomes prepared from bovine heart lipids | Homo sapiens |
| 0.000011 | - |
peptide transport rate, TAPL reconstituted in liposomes prepared from bovine brain lipids | Homo sapiens |
| 0.000023 | - |
peptide transport rate, TAPL reconstituted in liposomes prepared from bovine liver lipids | Homo sapiens |
| 0.000028 | - |
peptide transport rate | Homo sapiens |
| 0.000045 | - |
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, 100% phosphatidylcholine | Homo sapiens |
| 0.00007 | - |
peptide transport rate, TAPL reconstitution in liposomes composed of DOPC and 90% DOPS | Homo sapiens |
| 0.000075 | - |
peptide transport rate, TAPL reconstitution in liposomes of defined lipid composition, DOPC-DOPA, 9 to 1 | Homo sapiens |
| 0.000095 | - |
peptide transport rate, TAPL reconstitution in liposomes composed of DOPC and 10% DOPS | Homo sapiens |
| 0.000112 | - |
peptide transport rate | Homo sapiens |
| 0.000115 | - |
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, 70% phosphatidylcholine | Homo sapiens |
| 0.000135 | - |
peptide transport rate, TAPL reconstitution in liposomes of defined lipid composition, DOPC-DOPS, 9 to 1 | Homo sapiens |
| 0.000245 | - |
peptide transport rate, TAPL reconstitution in liposomes composed of DOPC and 70% DOPS | Homo sapiens |
| 0.00026 | - |
peptide transport rate, TAPL reconstitution in liposomes composed of DOPC and 30% DOPS | Homo sapiens |
| 0.00028 | - |
peptide transport rate | Homo sapiens |
| 0.000295 | - |
peptide transport rate | Homo sapiens |
| 0.00042 | - |
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids | Homo sapiens |
| 0.000423 | - |
peptide transport rate, TAPL reconstitution in liposomes composed of DOPC and 50% DOPS | Homo sapiens |
| 0.000477 | - |
peptide transport rate | Homo sapiens |
| 0.000505 | - |
peptide transport rate, TAPL reconstitution in Eschericha coli liposomes, for comparison | Homo sapiens |
| 0.000533 | - |
peptide transport rate | Homo sapiens |
| 0.000535 | - |
peptide transport rate, TAPL reconstitution in liposomes of defined lipid composition, for comparison in Eschericha coli liposomes | Homo sapiens |
| 0.00055 | - |
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, no phosphatidylcholine | Homo sapiens |
| 0.000673 | - |
peptide transport rate | Homo sapiens |
| 0.000715 | - |
peptide transport rate | Homo sapiens |
| 0.00127 | - |
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, 50% phosphatidylcholine | Homo sapiens |
| 0.00129 | - |
peptide transport rate | Homo sapiens |
| 0.00132 | - |
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, 10% phosphatidylcholine | Homo sapiens |
| 0.00168 | - |
peptide transport rate, TAPL reconstituted in liposomes prepared from Escherichia coli lipids, 30% phosphatidylcholine | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + CRYQKSTEL/in | peptide transport assay, fluorescein is coupled via the cysteine | Homo sapiens | ADP + phosphate + CRYQKSTEL/out | - |
? | |
| ATP + H2O + peptide/in | - |
Homo sapiens | ADP + phosphate + peptide/out | - |
? | |
| ATP + H2O + RCYQKSTEL/in | peptide transport assay, fluorescein is coupled via the cysteine | Homo sapiens | ADP + phosphate + RCYQKSTEL/out | - |
? | |
| ATP + H2O + RRCQKSTEL/in | peptide transport assay, fluorescein is coupled via the cysteine | Homo sapiens | ADP + phosphate + RRCQKSTEL/out | - |
? | |
| ATP + H2O + RRYCKSTEL/in | peptide transport assay, fluorescein is coupled via the cysteine | Homo sapiens | ADP + phosphate + RRYCKSTEL/out | - |
? | |
| ATP + H2O + RRYQCSTEL/in | peptide transport assay, fluorescein is coupled via the cysteine | Homo sapiens | ADP + phosphate + RRYQCSTEL/out | - |
? | |
| ATP + H2O + RRYQKCTEL/in | peptide transport assay, fluorescein is coupled via the cysteine | Homo sapiens | ADP + phosphate + RRYQKCTEL/out | - |
? | |
| ATP + H2O + RRYQKSCEL/in | peptide transport assay, fluorescein is coupled via the cysteine | Homo sapiens | ADP + phosphate + RRYQKSCEL/out | - |
? | |
| ATP + H2O + RRYQKSTCL/in | peptide transport assay, fluorescein is coupled via the cysteine | Homo sapiens | ADP + phosphate + RRYQKSTCL/out | - |
? | |
| ATP + H2O + RRYQKSTEC/in | peptide transport assay, fluorescein is coupled via the cysteine | Homo sapiens | ADP + phosphate + RRYQKSTEC/out | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ABCB9 | - |
Homo sapiens |
| lysosomal transport complex ABCB9 | - |
Homo sapiens |
| peptide-transporting ATPase | - |
Homo sapiens |
| TAPL | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
peptide transport assay | Homo sapiens |
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