Cloned (Comment) | Organism |
---|---|
human TAPL is cloned into pEGFP-N3 and pIRES2-EGFP resulting in TAPL with a C-terminal EGFP and TAPL co-translated EGFP, respectively, TAPL containing a C-terminal myc-tag is cloned into pcDNA3.1+, and into the retroviral vector pLPCX | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K545A/H699A | TAPL double mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
orthovanadate | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
monomer, determined by SDS-PAGE and immunoblotting | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + peptide/in | Homo sapiens | - |
ADP + phosphate + peptide/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
BRE-169 cell | TAP1-deficient skin fibroblast cell line | Homo sapiens | - |
Burkitt lymphoma cell | - |
Homo sapiens | - |
HEK-293T cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
monocyte | TAPL expression is strongly induced during differentiation of monocytes to dendritic cells and to macrophages | Homo sapiens | - |
STF1-169 cell | TAP2-deficient skin fibroblast cell line | Homo sapiens | - |
THP-1 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000022 | - |
peptide transport rate, K545A/H699A mutant, in the presence of 3 mM ATP | Homo sapiens |
0.00000022 | - |
peptide transport rate, wild type, in the presence of 3 mM ATP and 1 mM orthovanadate | Homo sapiens |
0.00000026 | - |
peptide transport rate, K545A/H699A mutant, in the presence of 3 mM ATP and 500 microM competitor peptide RRYQKSTEL | Homo sapiens |
0.00000026 | - |
peptide transport rate, wild type, in the presence of 3 mM AMP | Homo sapiens |
0.0000003 | - |
peptide transport rate, K545A/H699A mutant, in the presence of 3 mM ATP and 1 mM orthovanadate | Homo sapiens |
0.00000035 | - |
peptide transport rate, wild type, in the presence of 3 mM ATP and 500 microM competitor peptide RRYQKSTEL | Homo sapiens |
0.00000039 | - |
peptide transport rate, K545A/H699A mutant, in the presence of 3 mM AMP | Homo sapiens |
0.00000156 | - |
peptide transport rate, wild type, in the presence of 3 mM ATP | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + peptide/in | - |
Homo sapiens | ADP + phosphate + peptide/out | - |
? | |
ATP + H2O + RRYCKSTEL/in | substrate in peptide transport assay, fluorescein coupled via a cyteine residue | Homo sapiens | ADP + phosphate + RRYCKSTEL/out | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 70000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ABCB9 | - |
Homo sapiens |
peptide-transporting ATPase | - |
Homo sapiens |
TAP-like transporter | - |
Homo sapiens |
TAPL | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
peptide transport assay | Homo sapiens |