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Literature summary for 7.4.2.5 extracted from
- Biogenesis of functional antigenic peptide transporter TAP requires assembly of pre-existing TAP1 with newly synthesized TAP2 (2006), J. Biol. Chem., 281, 17545-17551.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pSP64 vector | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| TAP2DELTATM1 | mutant, amino acids 2-42 are removed | Rattus norvegicus |
| TAP2DELTATM2 | mutant, amino acids 2-88 are removed | Rattus norvegicus |
| TAP2DELTATM3 | mutant, amino acids 2-128 are removed | Rattus norvegicus |
| TAP2DELTATM4 | mutant, amino acids 2-185 are removed | Rattus norvegicus |
| TAP2DELTATM5 | mutant, amino acids 2-241 are removed | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | - |
Rattus norvegicus | 5789 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
TAP2, determined by SDS-PAGE | Rattus norvegicus |
| 72000 | - |
TAP1, determined by SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + peptide/in | Rattus norvegicus | - |
ADP + phosphate + peptide/out | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| T2 cell | human TAP-deficient lymphoblastoid cell, expressing rat wild-type TAP or single rat TAP1 or TAP2 chains | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + peptide/in | - |
Rattus norvegicus | ADP + phosphate + peptide/out | - |
? | |
| ATP + H2O + TVDNKTRYR/in | substrate in peptide transport assay, peptide is biotin-labeled at the lysine residue | Rattus norvegicus | ADP + phosphate + TVDNKTRYR/out | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 70000 (TAP1) + 1 * 72000 (TAP2), SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| peptide-transporting ATPase | - |
Rattus norvegicus |
| TAP | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
peptide transport assay | Rattus norvegicus |
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Release 2023.1 (January 2023)
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