Literature summary for 7.4.2.5 extracted from

Hou, J.M.; DLima, N.G.; Rigel, N.W.; Gibbons, H.S.; McCann, J.R.; Braunstein, M.; Teschke, C.M.
ATPase activity of Mycobacterium tuberculosis SecA1 and SecA2 proteins and its importance to SecA2 function in macrophages (2008), J. Bacteriol., 190, 4880-4887.

Search for more literature for 7.4.2.5

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli
into the pET29a vector for expression in Escherichia coli BL21DE3 cells	Mycobacterium tuberculosis
into the pET41b vector for expression in Escherichia coli BL21DE3 cells	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
K108R	mutant, defective in ATP binding and protein translocation in vitro, as well as biologically inactive in vivo	Escherichia coli
K115A	mutant, substitution of the conserved lysine in the Walker A motif eliminates ATP binding and affects the biological activity	Mycobacterium tuberculosis
K115R	mutant, substitution of the conserved lysine in the Walker A motif eliminates ATP binding and affects the biological activity	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	ATP hydrolysis rate 10.5 nmol phosphate/min/nmol SecA1	Mycobacterium tuberculosis
additional information	-	additional information	ATP hydrolysis rate SecA wild-type 6.1 nmol phosphate/min/nmol enzyme	Escherichia coli
additional information	-	additional information	ATP hydrolysis rate SecA2 wild-type 19.7, K115R mutant 25.0, K115A mutant 13.5 nmol Pi/min/nmol enzyme	Mycobacterium tuberculosis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Mycobacterium tuberculosis	5737	-
cytoplasm	-	Escherichia coli	5737	-
cytoplasmic membrane	-	Mycobacterium tuberculosis	-	-
cytoplasmic membrane	-	Escherichia coli	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
85400	-	monomer, predicted molecular mass, determined by SDS-PAGE	Mycobacterium tuberculosis
95000	-	determined by SDS-PAGE	Escherichia coli
101000	-	monomer, predicted molecular mass	Escherichia coli
106000	-	monomer, predicted molecular mass, determined by SDS-PAGE	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Mycobacterium tuberculosis	-	ADP + phosphate	-	ir
ATP + H2O	Escherichia coli	-	ADP + phosphate	-	ir
ATP + H2O	Mycobacterium tuberculosis H37Rv	-	ADP + phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P10408	-	-
Mycobacterium tuberculosis	P9WGP3	-	-
Mycobacterium tuberculosis	P9WGP5	-	-
Mycobacterium tuberculosis H37Rv	P9WGP3	-	-
Mycobacterium tuberculosis H37Rv	P9WGP5	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli
using a Blue-Sepharose CL-6B column	Mycobacterium tuberculosis

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Mycobacterium tuberculosis	ADP + phosphate	-	ir
ATP + H2O	-	Escherichia coli	ADP + phosphate	-	ir
ATP + H2O	-	Mycobacterium tuberculosis H37Rv	ADP + phosphate	-	ir

Synonyms

Synonyms	Comment	Organism
SecA	-	Escherichia coli
SecA ATPase	-	Escherichia coli
SecA1	-	Mycobacterium tuberculosis
SecA1 ATPase	-	Mycobacterium tuberculosis
SecA2	-	Mycobacterium tuberculosis
SecA2 ATPase	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	ATPase activity assay	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	ATPase activity assay	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)