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Literature summary for 7.4.2.5 extracted from
- Additional in vitro and in vivo evidence for SecA functioning as dimers in the membrane: dissociation into monomers is not essential for protein translocation in Escherichia coli (2008), J. Bacteriol., 190, 1413-1418.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pET5a vector for expression in Escherichia coli BL21DE3 cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| SecAA | tandem SecA | Escherichia coli |
| SecADELTA11/N95 | monomeric SecA derivative mutant, which lacks extreme terminal residues and shows in vitro and in vivo activities | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Escherichia coli | 5737 | - |
| cytoplasmic membrane | - |
Escherichia coli | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 93000 | - |
mutant SecAdelta11/N95, determined by SDS-PAGE | Escherichia coli |
| 97000 | - |
SecA, determined by SDS-PAGE | Escherichia coli |
| 200000 | - |
tandem SecAA, determined by SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P10408 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Q-Sepharose 26/10 anion-exchange column, a SP-Sepharose 16/5 cation exchange column, and a Superose 12 HR gel filtration column | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SecA | - |
Escherichia coli |
| SecA ATPase | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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