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Literature summary for 7.4.2.5 extracted from
- SecA2 is distinct from SecA in immunogenic specificity, subcellular distribution and requirement for membrane anchoring in Streptococcus parasanguis (2006), FEMS Microbiol. Lett., 264, 174-181.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pET-27b+ vector for expression in Escherichia coli BL21DE3 cells | Streptococcus parasanguinis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| SecA2DELTA1-990 | mutant, cloned into different plamids, construction of different strains | Streptococcus parasanguinis |
| SecA2DELTA1732-2397 7 | mutant, cloned into different plamids, construction of different strains | Streptococcus parasanguinis |
| SecA2DELTA1768-2028 | mutant, cloned into different plamids, construction of different strains | Streptococcus parasanguinis |
| SecA2DELTA991-1749 | mutant, cloned into different plamids, construction of different strains | Streptococcus parasanguinis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Streptococcus parasanguinis | 5737 | - |
| cytoplasmic membrane | - |
Streptococcus parasanguinis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
Streptococcus parasanguis strain VT1697, expressed from plasmid, deletion mutant, residues 1-330 are deleted | Streptococcus parasanguinis |
| 64000 | - |
Streptococcus parasanguis strain VT1574, expressed from chromosomal locus, deletion mutant, residues 331-583 are deleted, 83.6% in the membrane | Streptococcus parasanguinis |
| 64000 | - |
Streptococcus parasanguis strain VT1584, expressed from chromosomal locus, deletion mutant | Streptococcus parasanguinis |
| 65000 | - |
Streptococcus parasanguis strain VT1694, expressed from plasmid, deletion mutant, residues 578-797 are deleted, 76.6% in the membrane | Streptococcus parasanguinis |
| 79000 | - |
Streptococcus parasanguis strain VT1584, expressed from plasmid, deletion mutant, residues 678-779 are deleted, 68.0% in the membrane | Streptococcus parasanguinis |
| 81000 | - |
Streptococcus parasanguis strain VT1583, expressed from chromosomal locus, deletion mutant, residues 590-676 are deleted | Streptococcus parasanguinis |
| 81000 | - |
Streptococcus parasanguis strain VT1700, expressed from chromosomal locus, deletion mutant, residues 590-676 are deleted | Streptococcus parasanguinis |
| 81000 | - |
Streptococcus parasanguis strain VT1702, expressed from chromosomal locus, deletion mutant, residues 590-676 are deleted | Streptococcus parasanguinis |
| 82000 | - |
Streptococcus parasanguis strain VT1702, expressed from plasmid, deletion muatnt, 1-330 are deleted, GFP-tag, 84.4% in the membrane | Streptococcus parasanguinis |
| 92000 | - |
Streptococcus parasanguis strain FW213, expressed from chromosomal locus, full-length protein, 95.5% in the membrane | Streptococcus parasanguinis |
| 92000 | - |
Streptococcus parasanguis strain VT1694, expressed from chromosomal locus, full-length protein | Streptococcus parasanguinis |
| 92000 | - |
Streptococcus parasanguis strain VT1697, expressed from chromosomal locus, full-length protein | Streptococcus parasanguinis |
| 109000 | - |
Streptococcus parasanguis strain VT1700, expressed from plasmid, deletion mutant, residues 590-676 are deleted, GFP-tag, 86.8% in the membrane | Streptococcus parasanguinis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Streptococcus parasanguinis | - |
ADP + phosphate | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus parasanguinis | - |
- |
- |
| Streptococcus parasanguinis | A3CLD3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus parasanguinis |
| using a His Bind purification kit | Streptococcus parasanguinis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| SecA2-His6 protein is purified from inclusion bodies and refolded | Streptococcus parasanguinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Streptococcus parasanguinis | ADP + phosphate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SecA | - |
Streptococcus parasanguinis |
| SecA1 | - |
Streptococcus parasanguinis |
| SecA2 | - |
Streptococcus parasanguinis |
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