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Literature summary for 7.4.2.5 extracted from
- Cross-linked SecA dimers are not functional in protein translocation (2007), FEBS Lett., 581, 2616-2620.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | effect on mutant N95CC, a large stimulation of ATPase activity is only observed when both proOmpA and DTT are present | Escherichia coli |  |
| proOmpA | effect on mutant N95CC, a large stimulation of ATPase activity is only observed when both proOmpA and DTT are present | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N95 | truncated version of Escherichia coli SecA, the last 70 residues are lacking | Escherichia coli |
| N95CC | two cysteines are introduced into a truncated version of Escherichia coli SecA, at position 636 and 801, the last 70 residues are lacking, mutant is dimeric and fully functional | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.143 | - |
ATP | mutant N95CC treated with diamide, presence of DTT | Escherichia coli | |
| 0.263 | - |
ATP | mutant N95CC treated with diamide, absence of DTT | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | SecA ATPase | Escherichia coli | 5829 | - |
| inner membrane | SecY channel | Escherichia coli | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P10408 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the dimer, crosslinked by disulfide bridges, is inactive, the monomer is active | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chloroplast protein-transporting ATPase | - |
Escherichia coli |
| SecA | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
ATPase and translocation assay | Escherichia coli |
| 37 | - |
ATPase and translocation assay | Escherichia coli |
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