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Literature summary for 7.4.2.5 extracted from

  • Zaitseva, J.; Jenewein, S.; Wiedenmann, A.; Benabdelhak, H.; Holland, I.B.; Schmitt, L.
    Functional characterization and ATP-induced dimerization of the isolated ABC-domain of the haemolysin B transporter (2005), Biochemistry, 44, 9680-9690.
    View publication on PubMed
Search for more literature for 7.4.2.5

Protein Variants

Protein Variants Comment Organism
H662A inactive Escherichia coli
Y477W KM-value for ATP is 1.75fold higher than wild-type value. kcat for ATP is 4.3fold higher than wild-type value Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 ATP wild-type enzyme Escherichia coli
0.63
-
 ATP mutant enzyme Y477W Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + alpha-haemolysin/in
-
 Escherichia coli ADP + phosphate + alpha-haemolysin/out
-
 ?
additional information one intact nucleotide-binding domain within a dimer is sufficient for ATP hydrolysis. One ATP-binding site of the dimer is able to function independently of the hydrolytic capability of the neighboring ATP-binding site. Sequential mechanism of ATP hydrolysis in the intact HlyB transporter Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
dimer is the active form of the enzyme. ATP-promoted dimerization of HlyB-nucleotide binding domain might involve formation of two interconvertible forms: a readily dissociable active dimer and a more stable, reversibly inactive form of the dimer Escherichia coli

Synonyms

Synonyms Comment Organism
haemolysin B transporter
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.021
-
 ATP mutant enzyme Y477W Escherichia coli
0.093
-
 ATP wild-type enzyme Escherichia coli