Cloned (Comment) | Organism |
---|---|
recombinant production of the C-terminally His-tagged enzyme by Triticum aestivum germ lysates, co-expression with the proteins of the interactome of the TIM23 core complex | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Saccharomyces cerevisiae | preprotein import-driving ATPase activity of mtHsp70 | ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Triticum aestivum germ lysates by nickel affinity chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
ATP + H2O | preprotein import-driving ATPase activity of mtHsp70 | Saccharomyces cerevisiae | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mitochondrial heat shock protein 70 | - |
Saccharomyces cerevisiae |
mtHSP70 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | for the ATP-dependent translocation of preproteins into the aqueous matrix, TIM23CORE cooperates with the presequence translocase-associated import motor (PAM) that is composed of mitochondrial heat shock protein 70 (mtHsp70) and five co-chaperones that differentially regulate the import-driving ATPase activity of mtHsp70 and the assembly status of PAM: Tim44, Pam16, Pam17, Pam18, and Mge1. The biogenesis of presequence-carrying polytopic inner membrane proteins requires a close cooperation of the TIM23/PAM machinery with the export translocase Oxa1. Therefore, the TIM23 complex acts as an intricate multipurpose molecular machine in which the coupling of distinct partner proteins to TIM23CORE generates functional specificity | Saccharomyces cerevisiae |
additional information | quantitative analysis of mitochondrial protein complexes, overview | Saccharomyces cerevisiae |