Cloned (Comment) | Organism |
---|---|
cpHsc70-1 and cpHsc70-2 cDNAs are PCR amplified and cloned into NdeI and XhoI sites of pET28a and introduced into Escherichia coli BL21(DE3) | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a T-DNA insertion line for cpHsc70-1 DELTAcpHsc70-1 has variegated cotyledons, malformed leaves, growth retardation, impaired root growth and sensitivity to heat shock treatment. In addition, under stress conditions, the mutant exhibits unusual sepals, and malformed flowers | Arabidopsis thaliana |
additional information | cpHsc70-1/cpHsc70-2 double mutants are lethal | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | cpHsc70-1 and cpHsc70-2 are highly conserved proteins, imported into chloroplasts, analyzed by fluorescence microscopy | Arabidopsis thaliana | 9507 | - |
chloroplast stroma | YFP-fusion proteins of cpHsc70-1 and cpHsc70-2 are predominantly stromal, analyzed by fluorescence microscopy | Arabidopsis thaliana | 9570 | - |
thylakoid membrane | low level, analyzed by fluorescence microscopy | Arabidopsis thaliana | 42651 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
cpHsc70-1 | highly conserved, imported into chloroplasts | Arabidopsis thaliana |
cpHsc70-2 | highly conserved, imported into chloroplasts | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | expression patterns for cpHsc70-1 and cpHsc70-2 genes analyzed using the GUS (beta-gucuronidase) reporter gene. High Expression determined throughout seedlings, in all tissues of mature plants, unaltered in the drak or low light. Both genes are ubiquitously expressed at high levels, cpHsc70-1 slightly higher than cpHsc70-2, especially in roots | additional information |