Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GrpEL1 | the proposed nucleotide exchange factor of the human mtHsp70 machine. To access the nucleotide relase of human GrpEL1, a single turnover ATPase assay is performed to monitor the hydrolysis of the prebound mtHsp70-ATP complex of the wild-type protein in the presence of GrpEL1. Human GrpEL1 shows robust nucleotide exchange activity in the presence of excess unlabeled ATP, thus inhibiting the maximum ATP hydrolysis of wild-type mtHsp70 | Homo sapiens | |
human Hsp70 escort protein | human Hsp70 escort protein (Hep) possesses the unique ability to stimulate the ATPase activity of mtHsp70 as well as to prevent the aggregation of unfolded client proteins similar to J-proteins | Homo sapiens | |
J-protein | J-protein splice hTid-1L, larger isoform and hTid-1S, smaller isoform. At a morlar ratio of mtHsp70 to J-protein, a 3.2fold stimulation is observed with hTid-1L for wild-type protein. hTid-1S shows an 11fold stimulation for wild-type mtHp70 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
for purification of the His-tagged human mtHsp70, coexpression is carried out with yeast Hsp70 escort protein Hep in Escherichia coli BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y196A/N198A/D199A | YND mutant, shows 3fold elevated basal activity in comparison with wild-type. The mutant also shows a significant decrease in Hsp70 escort protein Hep stimulation as in comparison to the wild-type protein. The YND mutant also exhibits a decreased stimulation of the J-protein splice variant hTid-1S in comparison of the wild-type further supporting the mutually exclusive nature of Hep and J-protein interaction at the ATPase domain of human mtHsp70 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | to study the biochemical functions the components of the mtHsp70 machinery, Hsp70, J-protein, GrpE, and Hep are reconstituted | Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
the mitochondrial heat shock protein 70, mtHsp70 machinery components are highly conserved among eukaryotes, including humans | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged mtHsp70 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | binding of the client proteins to the SBD, substrate binding domain of the mtHsp70 brings a global change in the ATPase domain, thus enhancing the rate of ATP hydrolysis of Hsp70 | Homo sapiens | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mtHSP70 | - |
Homo sapiens |
mtHsp70-ATP complex | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, single turnover ATPase assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, single turnover ATPase assay | Homo sapiens |