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Literature summary for 7.4.2.3 extracted from

  • Goswami, A.V.; Chittoor, B.; DSilva, P.
    Understanding the functional interplay between mammalian mitochondrial Hsp70 chaperone machine components (2010), J. Biol. Chem., 285, 19472-19482.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
GrpEL1 the proposed nucleotide exchange factor of the human mtHsp70 machine. To access the nucleotide relase of human GrpEL1, a single turnover ATPase assay is performed to monitor the hydrolysis of the prebound mtHsp70-ATP complex of the wild-type protein in the presence of GrpEL1. Human GrpEL1 shows robust nucleotide exchange activity in the presence of excess unlabeled ATP, thus inhibiting the maximum ATP hydrolysis of wild-type mtHsp70 Homo sapiens
human Hsp70 escort protein human Hsp70 escort protein (Hep) possesses the unique ability to stimulate the ATPase activity of mtHsp70 as well as to prevent the aggregation of unfolded client proteins similar to J-proteins Homo sapiens
J-protein J-protein splice hTid-1L, larger isoform and hTid-1S, smaller isoform. At a morlar ratio of mtHsp70 to J-protein, a 3.2fold stimulation is observed with hTid-1L for wild-type protein. hTid-1S shows an 11fold stimulation for wild-type mtHp70 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
for purification of the His-tagged human mtHsp70, coexpression is carried out with yeast Hsp70 escort protein Hep in Escherichia coli BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y196A/N198A/D199A YND mutant, shows 3fold elevated basal activity in comparison with wild-type. The mutant also shows a significant decrease in Hsp70 escort protein Hep stimulation as in comparison to the wild-type protein. The YND mutant also exhibits a decreased stimulation of the J-protein splice variant hTid-1S in comparison of the wild-type further supporting the mutually exclusive nature of Hep and J-protein interaction at the ATPase domain of human mtHsp70 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion to study the biochemical functions the components of the mtHsp70 machinery, Hsp70, J-protein, GrpE, and Hep are reconstituted Homo sapiens 5739
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Organism

Organism UniProt Comment Textmining
Homo sapiens
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the mitochondrial heat shock protein 70, mtHsp70 machinery components are highly conserved among eukaryotes, including humans
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged mtHsp70 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O binding of the client proteins to the SBD, substrate binding domain of the mtHsp70 brings a global change in the ATPase domain, thus enhancing the rate of ATP hydrolysis of Hsp70 Homo sapiens ADP + phosphate
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Synonyms

Synonyms Comment Organism
mtHSP70
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Homo sapiens
mtHsp70-ATP complex
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Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
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assay at, single turnover ATPase assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
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assay at, single turnover ATPase assay Homo sapiens