Literature summary for 7.4.2.2 extracted from

Pernil, R.; Picossi, S.; Mariscal, V.; Herrero, A.; Flores, E.
ABC-type amino acid uptake transporters Bgt and N-II of Anabaena sp. strain PCC 7120 share an ATPase subunit and are expressed in vegetative cells and heterocysts (2008), Mol. Microbiol., 67, 1067-1080.

Cloned(Commentary)

Cloned (Comment)	Organism
ORFsalr4167 and alr3187 encode, respectively, an ATPase subunit, BgtA, and a composite protein bearing periplasmic substrate-binding and transmembrane domains, BgtB, of an ABC-type high-affinity basic amino acid uptake transporter Bgt. ORF alr4167 is clustered with ORFs alr4164, alr4165 and alr4166 that encode a periplasmic substrate-binding protein, NatF, and transmembrane proteins NatG and NatH respectively. The NatF, NatG, NatH and BgtA proteins constitute an ABC-type uptake transporter for acidic and neutral polar amino acids, N-II, Bgt and N-II transport systems thus share the ATPase subunit, BgtA. Structure of the strain PCC 7120 genomic regions carrying the bgtB gene and the natF-natG-natH-bgtA gene cluster, overview, expression of the Bgt and N-II transporters as GFP-tagged proteins in strain PCC 7120	Anabaena sp.

Cloned (Comment)

Organism

ORFsalr4167 and alr3187 encode, respectively, an ATPase subunit, BgtA, and a composite protein bearing periplasmic substrate-binding and transmembrane domains, BgtB, of an ABC-type high-affinity basic amino acid uptake transporter Bgt. ORF alr4167 is clustered with ORFs alr4164, alr4165 and alr4166 that encode a periplasmic substrate-binding protein, NatF, and transmembrane proteins NatG and NatH respectively. The NatF, NatG, NatH and BgtA proteins constitute an ABC-type uptake transporter for acidic and neutral polar amino acids, N-II, Bgt and N-II transport systems thus share the ATPase subunit, BgtA. Structure of the strain PCC 7120 genomic regions carrying the bgtB gene and the natF-natG-natH-bgtA gene cluster, overview, expression of the Bgt and N-II transporters as GFP-tagged proteins in strain PCC 7120

Anabaena sp.

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of deletion mutants of ORFs encoding BgtA, BgtB, NatF, NatG, and NatH, all showing altered substrate specificity compared to the wild-type enzyme, amino acid release and diazotrophy in the mutants, overview	Anabaena sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Anabaena sp.	16020	-
additional information	cellular and subcellular location of the Bgt and N-II transporters, in nitrate-grown filaments, the recombinant GFP-tagged proteins are observed in all the cells of the filament, with a somewhat higher signal in the periphery than in the centre of the cells and, especially, in the intercellular septa, overview	Anabaena sp.	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
ATP + H2O + alanine/out	Anabaena sp.	high activity	ADP + phosphate + alanine/in	-	?
ATP + H2O + arginine/out	Anabaena sp.	-	ADP + phosphate + arginine/in	-	?
ATP + H2O + glutamine/out	Anabaena sp.	-	ADP + phosphate + glutamine/in	-	?
ATP + H2O + glycine/out	Anabaena sp.	high activity	ADP + phosphate + glycine/in	-	?
ATP + H2O + histidine/out	Anabaena sp.	-	ADP + phosphate + histidine/in	-	?
ATP + H2O + leucine/out	Anabaena sp.	-	ADP + phosphate + leucine/in	-	?
ATP + H2O + lysine/out	Anabaena sp.	-	ADP + phosphate + lysine/in	-	?
ATP + H2O + nonpolar amino acid/out	Anabaena sp.	whereas Bgt is dispensable for diazotrophic growth, N-II appears to contribute together with N-I to the diazotrophic physiology of this cyanobacterium	ADP + phosphate + nonpolar amino acid/in	-	?
ATP + H2O + phenylalanine/out	Anabaena sp.	-	ADP + phosphate + phenylalanine/in	-	?
ATP + H2O + proline/out	Anabaena sp.	-	ADP + phosphate + proline/in	-	?
ATP + H2O + serine/out	Anabaena sp.	best substrate	ADP + phosphate + serine/in	-	?

Organism

Organism	UniProt	Comment	Textmining
Anabaena sp.	-	strain PCC 7120, ORFS salr4167 and alr3187 encoding BgtA and BgtB, and ORFs alr4167, alr4164, alr4165, and alr4166 encoding NatF, NatG, and NatH respectively	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
heterocyst	-	Anabaena sp.	-
additional information	cellular and subcellular location of the Bgt and N-II transporters, N-I that is expressed only in vegetative cells, while the Bgt and N-II systems are present in both vegetative cells and heterocysts, overview	Anabaena sp.	-
vegetative cell	-	Anabaena sp.	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + H2O + alanine/out	high activity	Anabaena sp.	ADP + phosphate + alanine/in	-	?
ATP + H2O + arginine/out	-	Anabaena sp.	ADP + phosphate + arginine/in	-	?
ATP + H2O + aspartate/out	low activity	Anabaena sp.	ADP + phosphate + aspartate/in	-	?
ATP + H2O + glutamate/out	low activity	Anabaena sp.	ADP + phosphate + glutamate/in	-	?
ATP + H2O + glutamine/out	-	Anabaena sp.	ADP + phosphate + glutamine/in	-	?
ATP + H2O + glycine/out	high activity	Anabaena sp.	ADP + phosphate + glycine/in	-	?
ATP + H2O + histidine/out	-	Anabaena sp.	ADP + phosphate + histidine/in	-	?
ATP + H2O + leucine/out	-	Anabaena sp.	ADP + phosphate + leucine/in	-	?
ATP + H2O + lysine/out	-	Anabaena sp.	ADP + phosphate + lysine/in	-	?
ATP + H2O + nonpolar amino acid/out	-	Anabaena sp.	ADP + phosphate + nonpolar amino acid/in	-	?
ATP + H2O + nonpolar amino acid/out	whereas Bgt is dispensable for diazotrophic growth, N-II appears to contribute together with N-I to the diazotrophic physiology of this cyanobacterium	Anabaena sp.	ADP + phosphate + nonpolar amino acid/in	-	?
ATP + H2O + phenylalanine/out	-	Anabaena sp.	ADP + phosphate + phenylalanine/in	-	?
ATP + H2O + proline/out	-	Anabaena sp.	ADP + phosphate + proline/in	-	?
ATP + H2O + serine/out	best substrate	Anabaena sp.	ADP + phosphate + serine/in	-	?
additional information	substrate specificity of wild-type and mutant strains, overview	Anabaena sp.	?	-	?

Subunits

Subunits	Comment	Organism
More	ORFsalr4167 and alr3187 encode, respectively, an ATPase subunit, BgtA, and a composite protein bearing periplasmic substrate-binding and transmembrane domains, BgtB, of an ABC-type high-affinity basic amino acid uptake transporter Bgt. ORF alr4167 is clustered with ORFs alr4164, alr4165 and alr4166 that encode a periplasmic substrate-binding protein, NatF, and transmembrane proteins NatG and NatH respectively. The NatF, NatG, NatH and BgtA proteins constitute an ABC-type uptake transporter for acidic and neutral polar amino acids, N-II, Bgt and N-II transport systems thus share the ATPase subunit, BgtA	Anabaena sp.

Synonyms

Synonyms	Comment	Organism
ABC-type high-affinity basic amino acid uptake transporter	-	Anabaena sp.
ATPase	-	Anabaena sp.
BGT	-	Anabaena sp.