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Literature summary for 7.4.2.11 extracted from

  • Kadaba, N.; Kaiser, J.; Johnson, E.; Lee, A.; Rees, D.
    The high-affinity E. coli methionine ABC transporter Structure and allosteric regulation (2008), Science, 321, 250-253 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Gold (lambdaDE3)cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 28-32% (w/v) PEG 400, 0.05 M Li2SO4, 0.2 M (NH4)2HPO4, 0.05-0.15 M (NH2)2SO4, and 0.1 M sodium citrate pH 4.2-4.5 Escherichia coli

Protein Variants

Protein Variants Comment Organism
E166Q inactive Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
L-methionine inhibits the ATPase activity of the enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + D-methionine-[methionine-binding protein][side 1] Escherichia coli
-
 ADP + phosphate + D-methionine[side 2] + [methionine-binding protein][side 1]
-
 ?
ATP + H2O + L-methionine-[methionine-binding protein][side 1] Escherichia coli
-
 ADP + phosphate + L-methionine[side 2] + [methionine-binding protein][side 1]
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P30750 and P31547 subunits metN and metI
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + D-methionine-[methionine-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + D-methionine[side 2] + [methionine-binding protein][side 1]
-
 ?
ATP + H2O + L-methionine-[methionine-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + L-methionine[side 2] + [methionine-binding protein][side 1]
-
 ?
ATP + H2O + L-selenomethionine-[methionine-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + L-selenomethionine[side 2] + [methionine-binding protein][side 1]
-
 ?

Synonyms

Synonyms Comment Organism
methionine ABC transporter
-
 Escherichia coli
methionine uptake transporter
-
 Escherichia coli
metI transmembrane subunit Escherichia coli
metN ATPase subunit Escherichia coli
MetNI
-
 Escherichia coli