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Literature summary for 7.4.2.1 extracted from

  • Merhi, A.; Gerard, N.; Lauwers, E.; Prevost, M.; Andre, B.
    Systematic mutational analysis of the intracellular regions of yeast Gap1 permease (2011), PLoS ONE, 6, e18457.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzymes in strains EK008 and EN121 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information generation of 47 mutant proteins reaching the plasma membrane normally, of two that are unstable and rapidly down-regulated even when the nitrogen source is poor. Six other mutants are totally inactive and another four, altered in a 16-amino-acid sequence in the N-terminal tail, are resistant to ammonium-induced down-regulation. Finally, a mutation in L6 causes missorting of Gap1 from the secretory pathway to the vacuole. Detailed overview Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane Gap1 comprises 12 transmembrane domains flanked by cytosol-facing N- and C-terminal tails Saccharomyces cerevisiae 5886
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0004 0.0387 activities of diverse mutant enzymes, overview, pH and temperature not specified in the publication Saccharomyces cerevisiae
0.0332
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wild-type Gap1 amino acid uptake activity, pH and temperature not specified in the publication Saccharomyces cerevisiae

Subunits

Subunits Comment Organism
More Gap1 comprises 12 transmembrane domains flanked by cytosol-facing N- and C-terminal tails. The N-terminal tail of Gap1 contains the acceptor lysines for ubiquitylation and its C-terminmal tail includes a sequence essential to exit from the endoplasmic reticulum Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
amino acid permease
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Saccharomyces cerevisiae
GAP1
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Saccharomyces cerevisiae
Gap1 permease
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Saccharomyces cerevisiae

Expression

Organism Comment Expression
Saccharomyces cerevisiae down-regulation of the mutant Gap1 permeases by ammonium, four gap1 mutants altered in the N-terminal tail resist ammonium-induced down-regulation, overview. Role of ubiquitin and acceptor lysines in constitutive down-regulation of mutant Gap1 proteins down