Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in strains EK008 and EN121 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of 47 mutant proteins reaching the plasma membrane normally, of two that are unstable and rapidly down-regulated even when the nitrogen source is poor. Six other mutants are totally inactive and another four, altered in a 16-amino-acid sequence in the N-terminal tail, are resistant to ammonium-induced down-regulation. Finally, a mutation in L6 causes missorting of Gap1 from the secretory pathway to the vacuole. Detailed overview | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | Gap1 comprises 12 transmembrane domains flanked by cytosol-facing N- and C-terminal tails | Saccharomyces cerevisiae | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0004 | 0.0387 | activities of diverse mutant enzymes, overview, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
0.0332 | - |
wild-type Gap1 amino acid uptake activity, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
Subunits | Comment | Organism |
---|---|---|
More | Gap1 comprises 12 transmembrane domains flanked by cytosol-facing N- and C-terminal tails. The N-terminal tail of Gap1 contains the acceptor lysines for ubiquitylation and its C-terminmal tail includes a sequence essential to exit from the endoplasmic reticulum | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
amino acid permease | - |
Saccharomyces cerevisiae |
GAP1 | - |
Saccharomyces cerevisiae |
Gap1 permease | - |
Saccharomyces cerevisiae |
Organism | Comment | Expression |
---|---|---|
Saccharomyces cerevisiae | down-regulation of the mutant Gap1 permeases by ammonium, four gap1 mutants altered in the N-terminal tail resist ammonium-induced down-regulation, overview. Role of ubiquitin and acceptor lysines in constitutive down-regulation of mutant Gap1 proteins | down |