BRENDA - Enzyme Database
show all sequences of 7.4.2.1

Transport activity-dependent intracellular sorting of the yeast general amino acid permease

Cain, N.E.; Kaiser, C.A.; Mol. Biol. Cell 22, 1919-1929 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of Gap1 mutants and of GFP-tagged Gap1p in a gap1DELTA car1DELTA sec6-4 strain that shows temperature sensitivity at 36°C
Saccharomyces cerevisiae
Engineering
Amino acid exchange
Commentary
Organism
A297V
site-directed mutagenesis, the catalytically inac­tive mutant does not respond to complex amino acid mixtures and constitutively sorts Gap1p to the plasma membrane. The mutant shows reduced activity with altered specificity compared to the wild-type enzyme and does not transport basic amino acids, its trafficking is also not regulated by these amino acids. The mutant has a specific defect in transport of positively charged amino acids
Saccharomyces cerevisiae
K9R/K16R
site-directed mutagenesis, the mutant shows reduced activity with altered specificity compared to the wild-type enzyme
Saccharomyces cerevisiae
K9R/K16R/A297V
site-directed mutagenesis, the mutant shows reduced activity with altered specificity compared to the wild-type enzyme
Saccharomyces cerevisiae
T106K
site-directed mutagenesis, the GTG motif mutant enzyme localized to the plasma membrane normally
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
citrulline
-
Saccharomyces cerevisiae
additional information
reversible inactivation of intrinsic Gap1p activity at the cell surface by amino acids requires active transport through Gap1p
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Saccharomyces cerevisiae
0.0127
-
glycine/out
Gap mutant K9R,K16R/A297V, pH and temperature not specified in the publication
Saccharomyces cerevisiae
0.02
-
glycine/out
Gap mutant K9R,K16R, pH and temperature not specified in the publication
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endosome
when amino acids are scarce Gap1p is sorted to the plasma membrane, whereas when amino acids are abundant Gap1p is sorted from the trans-Golgi through the multivesicular endosome and to the vacuole requiring direct recognition of transport substrates
Saccharomyces cerevisiae
5768
-
additional information
amino acids govern the propen­sity of Gap1p to recycle from the multivesicular endosome to the plasma membrane. Gap1p redistributes from the multivesicular endosome to the plasma membrane and that this recycling relies on the same machinery required for vesicular trafficking between the trans-Golgi and the plasma membrane
Saccharomyces cerevisiae
-
-
plasma membrane
when amino acids are scarce Gap1p is sorted to the plasma membrane, whereas when amino acids are abundant Gap1p is sorted from the trans-Golgi through the multivesicular endosome and to the vacuole requiring direct recognition of transport substrates
Saccharomyces cerevisiae
5886
-
vacuole
when amino acids are scarce Gap1p is sorted to the plasma membrane, whereas when amino acids are abundant Gap1p is sorted from the trans-Golgi through the multivesicular endosome and to the vacuole requiring direct recognition of transport substrates
Saccharomyces cerevisiae
5773
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + citrulline/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + citrulline/in
-
-
-
?
ATP + H2O + glycine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + glycine/in
-
-
-
?
ATP + H2O + L-alanine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-alanine/in
-
-
-
?
ATP + H2O + L-arginine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-arginine/in
-
-
-
?
ATP + H2O + L-glutamate/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-glutamate/in
-
-
-
?
ATP + H2O + L-lysine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-lysine/in
-
-
-
?
ATP + H2O + L-phenylalanine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-phenylalanine/in
-
-
-
?
ATP + H2O + L-threonine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-threonine/in
-
-
-
?
additional information
the GTG motif of Gap1p is involved in amino acid binding
720414
Saccharomyces cerevisiae
?
-
-
-
-
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.027
-
Gap mutant K9R,K16R/A297V, pH and temperature not specified in the publication
Saccharomyces cerevisiae
citrulline
0.0309
-
Gap mutant K9R,K16R, pH and temperature not specified in the publication
Saccharomyces cerevisiae
citrulline
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of Gap1 mutants and of GFP-tagged Gap1p in a gap1DELTA car1DELTA sec6-4 strain that shows temperature sensitivity at 36°C
Saccharomyces cerevisiae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A297V
site-directed mutagenesis, the catalytically inac­tive mutant does not respond to complex amino acid mixtures and constitutively sorts Gap1p to the plasma membrane. The mutant shows reduced activity with altered specificity compared to the wild-type enzyme and does not transport basic amino acids, its trafficking is also not regulated by these amino acids. The mutant has a specific defect in transport of positively charged amino acids
Saccharomyces cerevisiae
K9R/K16R
site-directed mutagenesis, the mutant shows reduced activity with altered specificity compared to the wild-type enzyme
Saccharomyces cerevisiae
K9R/K16R/A297V
site-directed mutagenesis, the mutant shows reduced activity with altered specificity compared to the wild-type enzyme
Saccharomyces cerevisiae
T106K
site-directed mutagenesis, the GTG motif mutant enzyme localized to the plasma membrane normally
Saccharomyces cerevisiae
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.027
-
Gap mutant K9R,K16R/A297V, pH and temperature not specified in the publication
Saccharomyces cerevisiae
citrulline
0.0309
-
Gap mutant K9R,K16R, pH and temperature not specified in the publication
Saccharomyces cerevisiae
citrulline
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
citrulline
-
Saccharomyces cerevisiae
additional information
reversible inactivation of intrinsic Gap1p activity at the cell surface by amino acids requires active transport through Gap1p
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Saccharomyces cerevisiae
0.0127
-
glycine/out
Gap mutant K9R,K16R/A297V, pH and temperature not specified in the publication
Saccharomyces cerevisiae
0.02
-
glycine/out
Gap mutant K9R,K16R, pH and temperature not specified in the publication
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endosome
when amino acids are scarce Gap1p is sorted to the plasma membrane, whereas when amino acids are abundant Gap1p is sorted from the trans-Golgi through the multivesicular endosome and to the vacuole requiring direct recognition of transport substrates
Saccharomyces cerevisiae
5768
-
additional information
amino acids govern the propen­sity of Gap1p to recycle from the multivesicular endosome to the plasma membrane. Gap1p redistributes from the multivesicular endosome to the plasma membrane and that this recycling relies on the same machinery required for vesicular trafficking between the trans-Golgi and the plasma membrane
Saccharomyces cerevisiae
-
-
plasma membrane
when amino acids are scarce Gap1p is sorted to the plasma membrane, whereas when amino acids are abundant Gap1p is sorted from the trans-Golgi through the multivesicular endosome and to the vacuole requiring direct recognition of transport substrates
Saccharomyces cerevisiae
5886
-
vacuole
when amino acids are scarce Gap1p is sorted to the plasma membrane, whereas when amino acids are abundant Gap1p is sorted from the trans-Golgi through the multivesicular endosome and to the vacuole requiring direct recognition of transport substrates
Saccharomyces cerevisiae
5773
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + citrulline/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + citrulline/in
-
-
-
?
ATP + H2O + glycine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + glycine/in
-
-
-
?
ATP + H2O + L-alanine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-alanine/in
-
-
-
?
ATP + H2O + L-arginine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-arginine/in
-
-
-
?
ATP + H2O + L-glutamate/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-glutamate/in
-
-
-
?
ATP + H2O + L-lysine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-lysine/in
-
-
-
?
ATP + H2O + L-phenylalanine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-phenylalanine/in
-
-
-
?
ATP + H2O + L-threonine/out
-
720414
Saccharomyces cerevisiae
ADP + phosphate + L-threonine/in
-
-
-
?
additional information
the GTG motif of Gap1p is involved in amino acid binding
720414
Saccharomyces cerevisiae
?
-
-
-
-
General Information
General Information
Commentary
Organism
malfunction
trafficking of Gap1p mutants with altered substrate specificity and transport activity, overview
Saccharomyces cerevisiae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
trafficking of Gap1p mutants with altered substrate specificity and transport activity, overview
Saccharomyces cerevisiae
Other publictions for EC 7.4.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746726
Huseinovic
Acetaminophen reduces the pro ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
Amino Acids
50
1377-1390
2018
-
-
-
-
-
1
-
-
-
-
-
2
-
60
-
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2
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1
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2
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-
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-
-
-
747810
Li
-
Effects of glutamate and aspa ...
Sus scrofa
Food Agric. Immunol.
29
675-687
2018
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
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-
1
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6
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3
-
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6
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
748336
Garneau
Function of pea amino acid pe ...
Pisum sativum
J. Exp. Bot.
69
5205-5219
2018
-
-
-
-
-
-
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-
1
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1
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3
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5
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1
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1
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1
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-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748827
Mueller
Amino acid permease 3 (aap3) ...
Leishmania amazonensis, Leishmania braziliensis, Leishmania donovani, Leishmania guyanensis, Leishmania infantum, Leishmania lainsoni, Leishmania major, Leishmania mexicana, Leishmania naiffi, Leishmania shawi, Leishmania tropica
Parasit. Vectors
11
421
2018
-
11
-
-
-
-
-
-
-
-
-
11
-
17
-
-
-
-
-
11
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
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-
11
-
-
-
-
-
11
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747962
Xia
Genome-wide identification an ...
Bemisia tabaci
Int. J. Biol. Sci.
13
735-747
2017
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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1
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-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748922
Ganeteg
Amino acid transporter mutant ...
Arabidopsis thaliana
Plant Cell Environ.
40
413-423
2017
-
-
-
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
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1
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
749046
Aoki
L-arginine availability and a ...
Leishmania amazonensis, Leishmania amazonensis MHOM/BR/1973/2269
PLoS Negl. Trop. Dis.
11
e0006025
2017
-
-
-
-
-
-
-
-
2
-
-
2
-
6
-
-
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-
2
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2
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2
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2
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2
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-
2
-
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-
-
-
-
-
-
-
1
-
-
1
-
-
747169
Price
Proton-dependent glutamine up ...
Acyrthosiphon pisum
Biochim. Biophys. Acta
1848
2085-2091
2015
-
-
1
-
-
-
-
1
1
-
-
1
-
8
-
-
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-
1
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1
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1
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1
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1
1
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1
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1
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1
-
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-
-
1
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-
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1
1
-
-
-
747866
Yang
The putative cationic amino a ...
Arabidopsis thaliana
Front. Plant Sci.
6
212
2015
-
-
-
-
-
-
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-
2
-
-
1
-
2
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1
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2
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1
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1
-
-
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-
-
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-
748099
Escudero
Functional dependence between ...
Nostoc sp. PCC 7120, Synechococcus elongatus
J. Bacteriol.
197
2721-2730
2015
-
-
1
-
-
-
-
-
-
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2
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11
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2
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1
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2
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2
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748108
Fulyani
Relative rates of amino acid ...
Lactococcus lactis, Lactococcus lactis NZ9000
J. Bacteriol.
198
477-485
2015
-
-
1
-
-
-
-
-
-
-
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9
-
-
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6
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1
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6
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748740
Boudko
Substrate specificity and tra ...
Aedes aegypti
Nat. Commun.
6
8546
2015
-
-
1
-
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-
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1
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1
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2
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9
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1
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1
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1
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9
-
-
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-
-
-
-
-
-
-
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-
-
-
-
749055
Pfannmueller
The general amino acid permea ...
Fusarium fujikuroi, Fusarium fujikuroi IMI58289
PLoS ONE
10
e0125487
2015
-
-
-
-
-
-
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3
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7
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4
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3
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4
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718559
Inbar
Lysine transporters in human t ...
Leishmania donovani, Trypanosoma cruzi
Amino Acids
42
347-360
2012
3
-
2
-
-
-
9
1
4
-
-
4
-
6
-
-
-
-
-
2
-
-
6
-
2
-
-
-
2
-
-
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3
-
2
-
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-
9
-
1
4
-
-
4
-
-
-
-
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2
-
-
6
-
2
-
-
-
2
-
-
-
-
1
1
-
-
-
719380
Kraidlova
The Candida albicans GAP gene ...
Candida albicans
Eukaryot. Cell
10
1219-1229
2011
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1
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1
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1
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-
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2
2
-
-
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719724
Ruiz
Identification of ArgP and Lrp ...
Escherichia coli
J. Bacteriol.
193
2536-2548
2011
-
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-
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1
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2
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1
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1
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1
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1
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1
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