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Literature summary for 7.4.2.1 extracted from

  • Cain, N.E.; Kaiser, C.A.
    Transport activity-dependent intracellular sorting of the yeast general amino acid permease (2011), Mol. Biol. Cell, 22, 1919-1929.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of Gap1 mutants and of GFP-tagged Gap1p in a gap1DELTA car1DELTA sec6-4 strain that shows temperature sensitivity at 36°C Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
A297V site-directed mutagenesis, the catalytically inac­tive mutant does not respond to complex amino acid mixtures and constitutively sorts Gap1p to the plasma membrane. The mutant shows reduced activity with altered specificity compared to the wild-type enzyme and does not transport basic amino acids, its trafficking is also not regulated by these amino acids. The mutant has a specific defect in transport of positively charged amino acids Saccharomyces cerevisiae
K9R/K16R site-directed mutagenesis, the mutant shows reduced activity with altered specificity compared to the wild-type enzyme Saccharomyces cerevisiae
K9R/K16R/A297V site-directed mutagenesis, the mutant shows reduced activity with altered specificity compared to the wild-type enzyme Saccharomyces cerevisiae
T106K site-directed mutagenesis, the GTG motif mutant enzyme localized to the plasma membrane normally Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
citrulline
-
 Saccharomyces cerevisiae
additional information reversible inactivation of intrinsic Gap1p activity at the cell surface by amino acids requires active transport through Gap1p Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Saccharomyces cerevisiae
0.0127
-
 glycine/out Gap mutant K9R,K16R/A297V, pH and temperature not specified in the publication Saccharomyces cerevisiae
0.02
-
 glycine/out Gap mutant K9R,K16R, pH and temperature not specified in the publication Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endosome when amino acids are scarce Gap1p is sorted to the plasma membrane, whereas when amino acids are abundant Gap1p is sorted from the trans-Golgi through the multivesicular endosome and to the vacuole requiring direct recognition of transport substrates Saccharomyces cerevisiae 5768
-
additional information amino acids govern the propen­sity of Gap1p to recycle from the multivesicular endosome to the plasma membrane. Gap1p redistributes from the multivesicular endosome to the plasma membrane and that this recycling relies on the same machinery required for vesicular trafficking between the trans-Golgi and the plasma membrane Saccharomyces cerevisiae
-
-
plasma membrane when amino acids are scarce Gap1p is sorted to the plasma membrane, whereas when amino acids are abundant Gap1p is sorted from the trans-Golgi through the multivesicular endosome and to the vacuole requiring direct recognition of transport substrates Saccharomyces cerevisiae 5886
-
vacuole when amino acids are scarce Gap1p is sorted to the plasma membrane, whereas when amino acids are abundant Gap1p is sorted from the trans-Golgi through the multivesicular endosome and to the vacuole requiring direct recognition of transport substrates Saccharomyces cerevisiae 5773
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + citrulline/out
-
 Saccharomyces cerevisiae ADP + phosphate + citrulline/in
-
 ?
ATP + H2O + glycine/out
-
 Saccharomyces cerevisiae ADP + phosphate + glycine/in
-
 ?
ATP + H2O + L-alanine/out
-
 Saccharomyces cerevisiae ADP + phosphate + L-alanine/in
-
 ?
ATP + H2O + L-arginine/out
-
 Saccharomyces cerevisiae ADP + phosphate + L-arginine/in
-
 ?
ATP + H2O + L-glutamate/out
-
 Saccharomyces cerevisiae ADP + phosphate + L-glutamate/in
-
 ?
ATP + H2O + L-lysine/out
-
 Saccharomyces cerevisiae ADP + phosphate + L-lysine/in
-
 ?
ATP + H2O + L-phenylalanine/out
-
 Saccharomyces cerevisiae ADP + phosphate + L-phenylalanine/in
-
 ?
ATP + H2O + L-threonine/out
-
 Saccharomyces cerevisiae ADP + phosphate + L-threonine/in
-
 ?
additional information the GTG motif of Gap1p is involved in amino acid binding Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
Gap1p
-
 Saccharomyces cerevisiae
general amino acid permease
-
 Saccharomyces cerevisiae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.027
-
 Gap mutant K9R,K16R/A297V, pH and temperature not specified in the publication Saccharomyces cerevisiae citrulline
0.0309
-
 Gap mutant K9R,K16R, pH and temperature not specified in the publication Saccharomyces cerevisiae citrulline

General Information

General Information Comment Organism
malfunction trafficking of Gap1p mutants with altered substrate specificity and transport activity, overview Saccharomyces cerevisiae