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Literature summary for 7.4.2.1 extracted from
- Purification and characterization of HisP, the ATP-binding subunit of a traffic ATPase (ABC transporter), the histidine permease of Salmonella typhimurium (1997), J. Biol. Chem., 272, 27745-27752.
General Stability
| General Stability | Organism |
|---|---|
| ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids is stable to freezing and thawing | Salmonella enterica subsp. enterica serovar Typhimurium |
| preparation diluted to 0.7 mg/ml protein and 4% glycerol is stable at 0°C for at least 1 h | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-O-(trinitrophenyl)adenosine 5'-triphosphate | ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 3'-O-(trinitrophenyl)adenosine 5'-triphosphate | ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| adenosine 5'-O-(3-thio)triphosphate | ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| adenylyl (beta,gamma-methylene)-diphosphate | ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| adenylyl (beta,gamma-methylene)-diphosphonate | ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| ADP | dimeric HisP protein with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| cardiolipin | ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| lysophosphatidic acid | ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| NEM | ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| phosphatidic acid | ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| phosphatidylglycerol | ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| phosphatidylserine | ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| vanadate | in contrast to the intact enzyme complex HisQMP2 the ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His is not inhibited | Salmonella enterica subsp. enterica serovar Typhimurium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | poor stimulatory activity on ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Co2+ | dimeric ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP at a rate comparable to that of the intact complex HisQMP2, requires a cation for activity, Co2+ is the best stimulator at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mg2+ | dimeric ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP at a rate comparable to that of the intact complex HisQMP2, requires a cation for activity, Co2+ and Mn2+ are more effective than Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mn2+ | dimeric ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP at a rate comparable to that of the intact complex HisQMP2, requires a cation for activity, Mn2+ is the best stimulator at concentrations above 1.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids, addition of a 6-histidine extension allows rapid and effective metal affinity purification | Salmonella enterica subsp. enterica serovar Typhimurium |
| nucleotide-binding subunit HisP | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids | Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5-10 mg/ml in storage buffer and in liquid nitrogen, ATP-binding subunit HisP with a carboxy-terminal extension of 8 amino acids is stable for at least several months | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + His/out | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + His/in | - |
? | |
| CTP + H2O + His/out | dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, 50% of the ATPase activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | CDP + phosphate + His/in | - |
? | |
| GTP + H2O + His/out | dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, 53% of the ATPase activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | GDP + phosphate + His/in | - |
? | |
| UTP + H2O + His/out | dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, 37% of the ATPase activity with ATP | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + phosphate + His/in | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
dimeric HisP protein with a carboxy-terminal extension of 8 amino acids - Leu-Glu-His-His-His-His-His-His - under optimal conditions hydrolyzes ATP to a rate comparable to that by the intact complex HisQMP2 | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 8 | pH 6.3: about 30% of maximal activity, pH 8.0: about 50% of maximal activity, dimeric HisP protein with a carboxy-terminal extension of 8 amino acids, Leu-Glu-His-His-His-His-His-His | Salmonella enterica subsp. enterica serovar Typhimurium |
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