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Literature summary for 7.3.2.7 extracted from

  • Lin, Y.F.; Walmsley, A.R.; Rosen, B.P.
    An arsenic metallochaperone for an arsenic detoxification pump (2006), Proc. Natl. Acad. Sci. USA, 103, 15617-15622.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.3.2.7

Activating Compound

Activating Compound Comment Organism Structure
ArsD encoded in the arsRDABC operon, the dimeric arsenic chaperone ArsD transfers trivalent metalloids to ArsA, the catalytic subunit of an As(III)-Sb(III) efflux pump. Interaction with ArsD increases the affinity of ArsA for arsenite and antimonite , thus increasing its ATPase activity at lower concentrations of arsenite 60fold, as well as for antimonite, and enhancing the rate of arsenite extrusion rendering cells resistant to environmental concentrations of arsenic, overview, cannot be replaced by DTT Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression of ArsA and ArsD wild-type and mutants in a Saccharomyces cerevisiae two-hybrid system for interaction analysis, overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
As3+ stimulates Escherichia coli
Mg2+ as MgATP2- Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + antimonite/in Escherichia coli the arsenic chaperone ArsD transfers trivalent metalloids to ArsA, the catalytic subunit of an As(III)-Sb(III) efflux pump. Interaction with ArsD increases the affinity of ArsA for antimonite, thus increasing its ATPase activity at lower concentrations of antimonite, regulatory mechanism, overview ADP + phosphate + antimonite/out
-
 ?
ATP + H2O + arsenite/in Escherichia coli the arsenic chaperone ArsD transfers trivalent metalloids to ArsA, the catalytic subunit of an As(III)-Sb(III) efflux pump. Interaction with ArsD increases the affinity of ArsA for arsenite, thus increasing its ATPase activity at lower concentrations of arsenite and enhancing the rate of arsenite extrusion rendering cells resistant to environmental concentrations of arsenic, regulatory mechanism, overview ADP + phosphate + arsenite/out
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
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 gene arsA, encoded in the arsRDABC operon
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + antimonite/in the arsenic chaperone ArsD transfers trivalent metalloids to ArsA, the catalytic subunit of an As(III)-Sb(III) efflux pump. Interaction with ArsD increases the affinity of ArsA for antimonite, thus increasing its ATPase activity at lower concentrations of antimonite, regulatory mechanism, overview Escherichia coli ADP + phosphate + antimonite/out
-
 ?
ATP + H2O + arsenite/in
-
 Escherichia coli ADP + phosphate + arsenite/out
-
 ?
ATP + H2O + arsenite/in the arsenic chaperone ArsD transfers trivalent metalloids to ArsA, the catalytic subunit of an As(III)-Sb(III) efflux pump. Interaction with ArsD increases the affinity of ArsA for arsenite, thus increasing its ATPase activity at lower concentrations of arsenite and enhancing the rate of arsenite extrusion rendering cells resistant to environmental concentrations of arsenic, regulatory mechanism, overview Escherichia coli ADP + phosphate + arsenite/out
-
 ?
additional information ArsA specifically interacts with ArsD but not with ArsR or ArsC Escherichia coli ?
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 ?

Synonyms

Synonyms Comment Organism
arsenic detoxification pump
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 Escherichia coli